Electroblotting is a technique to immboilise proteins or nucleic acid seperation on a solid membrane support. Samples are then detected using specific antibodies, ligands or nucleic acid probes that bind individual proteins or nucleic acid sequences. This allows identification, quantification or interaction’s study of proteins and nucleic acid from various samples, and makes it a powerful technique in proteomics and genomics.

Cleaver Scientific offers four types of systems: the modular electroblotters, tank transfers systems, semi-dry transfer systems and microfiltration (dot and slot blotting). The most well known application of electroblotting is the western blot, when proteins are immobilised on nitrocellulose or PVDF membranes. Protein abundance can then be quantified by staining with colorimetric dyes or protein specific antibodies. We supply a range of units suitable for western blotting of both precast size gels and large format polyacrylamide gels.

The Mini omniPAGE Sub Blot systems are complete stand-alone units for
submarine blotting. Each unit comprises an omniPAGE tank and lid,
electroblotting insert, and cassettes and fibre pads. The versatile
design of the omniPAGE tank and lid allows the unit to be easily adapted
for vertical PAGE or IEF with capillary tube gels, using the relevant
optional insert. Other features include:

• Multi-transfer capability for 4 Mini gels in the Mini system
• Complete run-time flexibility from overnight to as short as a couple of hours
• Cool pack for environmentally friendly low-cost cooling during high-intensity transfers
• Open design, rigid cassettes maximise current transfer and eliminate shadow band formation
• Cassettes colour-coded prevent polarity reversal to ensure transfer in the correct orientation

The Maxi format omniPAGE Sub Blot system is a complete stand-alone unit for
submarine blotting. Each unit comprises an omniPAGE tank and lid,
electroblotting insert, and cassettes and fibre pads. The versatile
design of the omniPAGE tank and lid allows the unit to be easily adapted
for vertical PAGE or IEF with capillary tube gels, using the relevant
optional insert. Other features include:

• Multi-transfer capability for up to 4 Maxi gels, 8 Mini Wide and 16 Mini gels
• Complete run-time flexibility from overnight to as short as a couple of hours
• Cool pack for environmentally friendly low-cost cooling during high-intensity transfers
• Open design, rigid cassettes maximise current transfer and eliminate shadow band formation
• Cassettes colour-coded prevent polarity reversal to ensure transfer in the correct orientation

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