Vivaspin 500, 3 kDa MWCO PES

Overview
Vivaspin™ ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples.

•Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss, as well as pH compatibility over a broad range – pH 1 to 9.
•Sample protection: Patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
•Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
•High flow rates: And minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
•Minimal protein adsorption: Thanks to the Vivaspin™ polycarbonate tube design.
•Wide selection: Vivaspin sample concentrators are suitable for sample volumes ranging from 100 µL to 20 mL, with a range of molecular weight cut-off (MWCO) values from Mr 3000 to 100 000.


Spin column protein concentration – how does it work?
Membrane spin columns such as Vivaspin combine the principle of size exclusion with centrifugation to achieve rapid concentration and high recovery of target biomolecules in a single tube. An upper compartment containing sample is separated from the lower compartment by a semipermeable membrane with an appropriate MWCO value chosen to exclude molecules of interest. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The Vivaspin column design permits protocols for one-step biomolecule concentration, rapid sample recovery (reverse spin protocol), desalting, and buffer exchange.