{"product_id":"vitafast®-vitamin-b6-pyridoxin-en","title":"VitaFast® Vitamin B6 (Pyridoxin)","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nThe VitaFast® Vitamin B6 (Pyridoxin) microtiter plate test is a microbiological method for the quantitative determination of total vitamin B6 (added and natural vitamin B6) in food, animal feed and in pharmaceutical products. The microbiological test system is in accordance with international norms.\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: ''- Liquid samples (added vitamin B6): enzymatic treatment, extraction, sterile filtration and dilution.\u003cbr\u003e\u003cbr\u003e\r\n- Solid samples (added vitamin B6): sample homogenization, enzymatic treatment, extraction, centrifugation and dilution. \u003cbr\u003e\u003cbr\u003e\r\n- Liquid and solid samples (added and native vitamin B6): sample homogenization, enzymatic treatment, extraction, centrifugation and dilution.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eIncubation time: 44 - 48 h in the dark at 30 °C (86 °F)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): 0.0002 mg \/ 100 g (ml)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOQ (Limit of quantification): 0.002 mg \/ 100 g (ml)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eValidated matrices: Multivitamin juice, fitness drink, fruit gums, candies, pills, capsules, vitamin mixes, cereals, baby food, bread, flour, meat, meat products, milk products, yeast, yeast products.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eDetected analyte: Pyridoxin concentration\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eEvaluation: Microtiter plate photometer 610-630 nm (alternativ 540-550 nm)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: VitaFast® Vitamin B12 (Cyanocobalamin) \u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nVitamin B6 is extracted from a homogenized sample and the extract is diluted. The diluted extract and standards are applied to the wells of a microtiter plate coated with \u003cem\u003eSaccharomyces cerevisiae\u003c\/em\u003e. The growth of the bacteria is dependent on the vitamin content of the sample, or standard. \u003cem\u003eSaccharomyces cerevisiae\u003c\/em\u003e belongs to the yeast bacteria, and has an absolute growth requirement for the vitamin B6. Thus, this organism is ideal for quantification of pyridoxin in food and dietary supplements. As a group, the yeast bacteria are good candidates for vitamin analysis due to their genetic stability (resistance to undergo mutation), simple metabolic requirements, ease of maintenance and general safety as a non-pathogenic organism.\r\n\u003cbr\u003e\u003ch3\u003eBenefits:\u003c\/h3\u003e\r\n\u003cul\u003e\r\n \t\u003cli\u003eThe test system is very robust and well suited for routine analysis.\u003c\/li\u003e\r\n \t\u003cli\u003eThe VitaFast® test kits feature uniform procedures across the product line.\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657899401507,"sku":"RBIOP1008","price":769.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/P1008.jpg?v=1779863615","url":"https:\/\/onelab.co.nz\/products\/vitafast%c2%ae-vitamin-b6-pyridoxin-en","provider":"Onelab","version":"1.0","type":"link"}