{"product_id":"vitafast®-vitamin-b1-thiamin-en","title":"VitaFast® Vitamin B1 (Thiamin)","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nThe VitaFast® Vitamin B1 (Thiamine) microtiter plate test is a microbiological method for the quantitative determination of total vitamin B1 (added and natural vitamin B1) in food, animal feed and in pharmaceutical products. The microbiological test system is in accordance with international norms.\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: ''- Liquid samples (added vitamin B1): sterile filtration and dilution.\u003cbr\u003e\u003cbr\u003e\r\n- Solid samples (added vitamin B1): sample homogenization, extraction, centrifugation and dilution.\u003cbr\u003e\u003cbr\u003e\r\n- Liquid and solid samples (added and native vitamin B1): sample homogenization, enzymatic treatment, extraction, centrifugation and dilution.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eIncubation time: 44 - 48 h in the dark at 37 °C (98.6 °F)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): 0.008 µg \/ 100 g (ml)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOQ (Limit of quantification): 0.012 mg \/ 100 g (ml)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eValidated matrices: Multivitamin juice, fitness drink, fruit gums, candies, pills, capsules, vitamin mixes, cereals, baby food, bread, flour, meat, meat products, milk products, yeast, yeast products.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eDetected analyte: Thiamin concentration (thiaminhydrochlorid)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eEvaluation: Microtiter plate photometer 610-630 nm (alternativ 540-550 nm)\u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nVitamin B1 is extracted from a homogenized sample and the extract is diluted. The diluted extract and standards are applied to the wells of a microtiter plate coated with\u003cem\u003e Lactobacillus fermentum\u003c\/em\u003e. The growth of the bacteria is dependent on the vitamin content of the sample, or standard. \u003cem\u003eLactobacillus fermentum\u003c\/em\u003e belongs to the lactic acid bacteria, and has an absolute growth requirement for the vitamin B1. Thus, this organism is ideal for quantification of thiamin in food and dietary supplements. As a group, the lactic acid bacteria are good candidates for vitamin analysis due to their genetic stability (resistance to undergo mutation), simple metabolic requirements, ease of maintenance and general safety as a non-pathogenic organism.\r\n\u003cbr\u003e\u003ch3\u003eBenefits:\u003c\/h3\u003e\r\n\u003cul\u003e\r\n \t\u003cli\u003eThe test system is very robust and well suited for routine analysis.\u003c\/li\u003e\r\n \t\u003cli\u003eThe VitaFast® test kits feature uniform procedures across the product line.\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657906839843,"sku":"RBIOP1006","price":769.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/P1006.jpg?v=1779864056","url":"https:\/\/onelab.co.nz\/products\/vitafast%c2%ae-vitamin-b1-thiamin-en","provider":"Onelab","version":"1.0","type":"link"}