VitaFast® Vitamin C (L-Ascorbic Acid)

Intended use:

VitaFast® Vitamin C (L-Ascorbic Acid) is a test in microtiter plate format for the quantitative determination of vitamin C (L-ascorbic acid) in foods, pharmaceutical products and other sample material. Furthermore the total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid) can be detected (see application note).

Specifications:

• Test format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)


• Sample preparation: Homogenisation, extraction, filtration, dilution,


• Incubation time: Step 1 = 20 min in the dark at 20-25 °C, Step 2 = 30 min in the dark at 20-25 °C.


• LOD (Detection limit): 7.8 mg / 100 g (ml)
• LOQ (Limit of quantification): 7.8 mg / 100 g (ml)


• Validated matrices: Fruit gums, multivitamin effervescent tablets, Iron and vitamin C tablets.


• Available application notes: Total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid)


• Detected analyte: Ascorbic acid concentration


• Evaluation: Microtiter plate photometer 550-570 nm


• Related products: VitaFast® Folic Acid |VitaFast® Vitamin B12 (Cyanocobalamin) |VitaFast® Vitamin B7 (Biotin)

General Information:

Ascorbic acid is a clear, odourless, cristalline solid substance which has a sour flavour. There are four stereoisomeric variants but only L-(+)-ascorbic acid (also referred to as vitamin C) is biologically active. Its vitamin activity is the most relevant property of L-ascorbic acid: humans cannot produce vitamin C and require it as part of their nutrition. A deficiency can result in the manifestation of scurvy. L-ascorbic acid and its salt, the anion L-ascorbate, will hereinafter be referred to as ascorbic acid and ascorbate, respectively.

Benefits:

• The method is robust and suitable for the routine determination of the ascorbic acid content in a multitude of foodstuffs and beverages.
• This test has very low detection and quantification limit.