{"product_id":"surefood®-gmo-screen-4plex-bar-pat-cryiab-iac-ctp2-cp4-epsps","title":"SureFood® GMO SCREEN 4plex BAR\/PAT\/CryIAb\/IAc\/ CTP2:CP4 EPSPS","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nThe SureFood® GMO SCREEN 4plex BAR\/PAT\/CryIAb\/IAc\/CTP2:CP4 EPSPS is a multiplex real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified DNA - sequences.\r\n\u003cul\u003e\r\n \t\u003cli\u003ePhosphinothricin-Acetyltransferase gene (BAR) from \u003cem\u003eStreptomyces hygroscopius\u003c\/em\u003e\n\u003c\/li\u003e\r\n \t\u003cli\u003egenetically engineered CryIAb-DNA-sequences and CryIAb\/Ac-fusion gene sequences\u003c\/li\u003e\r\n \t\u003cli\u003ePhosphinothricin-Acetyltransferase gene (PAT) from \u003cem\u003eStreptomyces viridiochromogenes\u003c\/em\u003e\n\u003c\/li\u003e\r\n \t\u003cli\u003ethe transition from CTP2 (Chloroplast-Transpeptide-signal sequence from \u003cem\u003eArabidopsis thaliana\u003c\/em\u003e) to herbicide tolerance-gene CP4 EPSPS (5-Enolpyruvylshikimat-3-Phosphat Syntheses gene from \u003cem\u003eAgrobacterium tumefaciens\u003c\/em\u003e strain CP4)\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\nThe detections are according to the validated methods of the European Commission or the official collection of detection methods of §64 German food law.\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: 100 reactions\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended. SureFood® PREP Add On is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): The SureFood® GMO SCREEN 4plex BAR\/PAT\/CryIAb\/IAc\/CTP2:CP4 EPSPS real-time PCR has a limit of detection of \u0026lt; 5 DNA copies. This is equivalent to a limit of detection of \u0026lt; 0.01 % based on the genetically modified organisms MON87751 Soya (CryIAb\/IAc), DAS-81419-2 Soya (PAT), NK603 Corn (CTP2:CP4 EPSPS) and Bt176 Corn (BAR).\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAccessories: SureFood® PREP Basic |SureFood® PREP Advanced |SureFood® PREP Add-On|SureFast® Mag PREP Food\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: SureFood® GMO SCREEN 4plex BAR\/NPTII\/PAT\/CTP2:CP4 EPSPS |SureFood® GMO SCREEN 4plex 35S\/NOS\/FMV\/IAC |RIDA®CYCLER|TANBead Maelstrom™ 4800\u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nThe real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC\/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657891275043,"sku":"RBIOS2128","price":1828.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/S2128.jpg?v=1779863278","url":"https:\/\/onelab.co.nz\/products\/surefood%c2%ae-gmo-screen-4plex-bar-pat-cryiab-iac-ctp2-cp4-epsps","provider":"Onelab","version":"1.0","type":"link"}