SureFood® GMO QUANT RoundUp Ready Soya

Intended use:

SureFood® GMO QUANT Roundup Ready Soya (RR soya) is a real-time PCR for the detection of the relative RR soya DNA amount to the total soya DNA content. Therefore, the kit contains two PCR systems, one for detection of a RR soya specific gene (Roundup Ready™ soya, GTS 40-3-2, OECD unique identifier MON-Ø4Ø32-6) and one for the detection of a soya gene (reference gene).

Specifications:

• Test format: 2x50 reactions


• Sample preparation: For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.


• LOD (Detection limit): The Roundup Ready Soya PCR has a limit of detection of = 5 DNA-copies. This is equivalent to approx. 0.01 % for unprocessed soybean. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.


• LOQ (Limit of quantification): The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for RR soya DNA is 0.1 %.


• Accessories: SureFood® PREP Basic |SureFood® PREP Add-On|SureFood® PREP Advanced |SureFast® Mag PREP Food


• Related products: RIDA®CYCLER|TANBead Maelstrom™ 4800|SureFood® GMO QUANT 35S Soya |SureFood® GMO QUANT RR2Y Soya

General Information:

The real-time PCR assay can be used with established real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96, Bio-Rad CFX96, Bio-Rad CFX Opus, R-Biopharm RIDA®CYCLER and Agilent AriaDx.