{"product_id":"surefood®-gmo-quant-gt73-canola-en","title":"SureFood® GMO QUANT GT73 Canola","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nSureFood® GMO QUANT GT73 Canola is a real-time PCR for the detection of the relative GT73 canola DNA amount to the total canola DNA content. Therefore, the kit contains two PCR systems, one for detection of a GT73 canola specific gene (OECD unique identifier MON-ØØØ73-7) and one for the detection of a canola gene (reference gene).\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: 2 x 50 reactions\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): The GT73 Canola PCR has a limit of detection of = 5 DNA-copies. This is equivalent to approx. 0.01 % for unprocessed canola grain.\r\nThe assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOQ (Limit of quantification): The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for GT73 canola DNA is 0.1 %.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eCross reactivity: \u003cp\u003eCross reactivity was observed with DNA extracts from \u003cem\u003eBrassicacea\u003c\/em\u003e family. That means that quantification is only possible for samples in which canola material is known to be the only \u003cem\u003eBrassicacea\u003c\/em\u003e type. Therefore the GT73 canola quantification in a mustard sample is not possible.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAccessories: SureFood® PREP Basic |SureFood® PREP Add-On|SureFood® PREP Advanced |SureFast® Mag PREP Food\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: RIDA®CYCLER|TANBead Maelstrom™ 4800\u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nThe real-time PCR assay can be used with established real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Bio-Rad CFX96 Dx, Bio-Rad CFX96 Opus and Agilent AriaDx.","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657905135907,"sku":"RBIOS2061","price":1683.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/S2061.jpg?v=1779863959","url":"https:\/\/onelab.co.nz\/products\/surefood%c2%ae-gmo-quant-gt73-canola-en","provider":"Onelab","version":"1.0","type":"link"}