{"product_id":"surefood®-animal-quant-beef-en","title":"SureFood® ANIMAL QUANT Beef (en)","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nThe test detects the relative beef DNA amount (\u003cem\u003eBos taurus\u003c\/em\u003e) to the total animal DNA content (amnions) in meat samples. Therefore the kit contains two PCR systems, one for detection of a beef specific gene (Beef detection gene) and one for the detection of an animal gene (Reference gene).\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: 2 x 50 reactions\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: For DNA-preparation of meat samples the use of SureFood® PREP Basic (Art. No. S1052) is recommended.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): The beef specific PCR has a limit of detection of = 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content. The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for beef DNA is 0.04 %.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eCross reactivity: \u003cp\u003eCross reactivity was observed with DNA extracts from Bison sp. (100 %) and Water buffalo (\u003cem\u003eBubalis arnee\u003c\/em\u003e) with 0.6%.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAccessories: SureFood® PREP Basic \u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: RIDA®CYCLER|SureFood ® ANIMAL  ID  4plex  Beef\/Horse\/Pork+IAAC|SureFood® ANIMAL ID 3plex Water Buffalo\/Beef+IAAC|SureFood® ANIMAL ID 4plex Beef\/Sheep\/Goat+IAAC |SureFood® ANIMAL ID Beef IAAC \u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nThe real-time PCR assay can be used with established real-time PCR instruments. The technical validation of instruments was performed on Agilent Mx3005P, Applied Biosystems 7500, Bio-Rad CFX96, Roche LightCycler® 2.0, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657897500963,"sku":"RBIOS1010","price":1683.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/S1010.jpg?v=1779863581","url":"https:\/\/onelab.co.nz\/products\/surefood%c2%ae-animal-quant-beef-en","provider":"Onelab","version":"1.0","type":"link"}