SureFood® GMO SCREEN P35S:BAR Rice

Intended use:

The SureFood® GMO SCREEN P35S:BAR Rice is a real-time PCR for the direct, qualitative detection of a specific P35S:BAR constructDNA sequence of P35S:BAR. The LibertyLink 601 (LL601 - OECD unique identifier BCS-OSØØ3-7) Rice and the LibertyLink 62 (LL62 - OECD unique identifier ACS-OSØØ2-5) Rice contains this genetically construct but it is also present in other GMOs as StarLink Corn and Bt176 Corn. Additionally, the kit contains a reference PCR system for the detection of Rice. The specific detection is according to the validated method of the European Commission ("Report on the verification of a construct-specific detection method for identification of Rice GM-events containing P35S:BAR using a real-time PCR assay" from the Joint Research Centre, the Community Reference Laboratory).

Specifications:

• Test format: 100 reactions, whereas 50 reactions are used for detection of the reference gene.


• Sample preparation: For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.


• LOD (Detection limit): The P35S:BAR PCR has a limit of detection of = 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed Rice grain. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.


• Accessories: SureFood® PREP Basic |SureFood® PREP Advanced |SureFast® Mag PREP Food|SureFood® PREP Add-On


• Related products: RIDA®CYCLER|TANBead Maelstrom™ 4800|SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS |SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS|SureFood® GMO SCREEN P35S:BAR Rice |SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, LTF MyGo Pro, Agilent AriaDx and Agilent Mx3005P.