SureFood® GMO ID Bt63 Rice

Intended use:

The SureFood® GMO ID Bt63 Rice is a real-time PCR for the direct, qualitative detection of a specific genetically modified Bt63 rice DNA sequence. Therefore, the kit contains a construct specific real-time PCR system for the Bt63 rice (OECD unique identifier HZU-HHØØ1-9). The specific detection is according to method published by: D. Maede et al.; European Food Research and Technology, 224:271-278 (2006) and therefore according to Commission decision 2008/289/EC.

Specifications:

• Test format: 2 x 50 reactions


• Sample preparation: For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.


• LOD (Detection limit): The SureFood® GMO ID Bt63 Rice assay has a limit of detection of = 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed rice grain. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.


• Accessories: SureFood® PREP Basic |SureFood® PREP Advanced |SureFast® Mag PREP Food


• Related products: RIDA®CYCLER|TANBead Maelstrom™ 4800|SureFood® GMO ID DAS-40278-9 Corn

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Roche LightCycler® 1.5, Eppendorf realplex, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER and Agilent Mx3005P.