SureFood® GMO ID 4plex Soya I

Intended use:

The multiplex test SureFood® GMO ID 4plex Soya I detects the following DNA-sequences of soya:

• FAM-channel: MON87708 Soja (OECD Bezeichnung MON-877Ø8-9)
• FAM-channel: CV127 Soja (OECD Bezeichnung BPS-CV127-9)
• VIC-channel: DP305423 Soja (OECD Bezeichnung DP-3Ø5423-1)
• ROX-channel: MON87701 Soja (OECD Bezeichnung MON877Ø1-2)
• Cy5-channel: MON87769 Soja (OECD Bezeichnung MON-87769-7)

The event specific detection is according to the official method of the European Commission.  

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.


• LOD (Detection limit): The SureFood® GMO ID 4plex Soya I real-time PCR has a limit of detection of <5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed soybeans.


• Accessories: SureFood® PREP Basic |SureFood® PREP Advanced |SureFast® Mag PREP Food|SureFood® PREP Add-On


• Related products: SureFood® GMO QUANT RoundUp Ready Soya |SureFood® GMO QUANT RR2Y Soya |SureFood® GMO QUANT 35S Soya |RIDA®CYCLER|TANBead Maelstrom™ 4800

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER and Agilent Mx3005P.