SureFood® GMO ID 4plex Canola II

Intended use:

The multiplex real-time PCR test detects the following DNA-sequences of genetically modified canola: - FAM channel: MON88302 canola (OECD unique identifier MON-883Ø2-9) - VIC channel: Canola - ROX channel: DP73496 canola (OECD unique identifier DP-Ø73496-4) - Cy5 channel: RF3 canola (OECD unique identifier ACS-BNØØ3-6) The detections are according to the validated method of the European Commission.

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. Nr. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.


• LOD (Detection limit): The SureFood® GMO ID 4plex Canola II real-time PCR has a limit of detection of < 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed canola grain.


• Accessories: SureFood® PREP Basic |SureFood® PREP Advanced |SureFood® PREP Add-On|SureFast® Mag PREP Food


• Related products: SureFood® GMO ID 4plex Canola I |SureFood® GMO QUANT GT73 Canola |RIDA®CYCLER|TANBead Maelstrom™ 4800

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II and R-Biopharm RIDA®CYCLER.