SureFood® GMO ID 4plex Canola I

Intended use:

The multiplex test detects the following DNA sequences of genetically modified canola:

• FAM channel: MS8 canola (OECD unique identifier ACS-BNØØ5-8)
• ROX channel: GT73 canola (OECD unique identifier MON-ØØØ73-7)
• Cy5 channel: T45 canola (OECD unique identifier ACS-BNØØ8-2)

The event specific detection is according to the official method of the European Commission. Each reaction contains an internal amplification control.  

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.


• LOD (Detection limit): The SureFood® GMO ID 4plex Canola I assay has a limit of detection of ca. 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed canola grain.


• Accessories: SureFood® PREP Basic |SureFood® PREP Advanced |SureFast® Mag PREP Food|SureFood® PREP Add-On


• Related products: SureFood® GMO QUANT GT73 Canola |RIDA®CYCLER|TANBead Maelstrom™ 4800|SureFood® GMO ID 4plex Canola II

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER und Agilent Mx3005P.