SureFood® ANIMAL ID Chicken IAAC (en)

Intended use:

The test detects chicken (Gallus gallus) DNA. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).  

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.


• LOD (Detection limit): The SureFood® ANIMAL ID Chicken IAAC real-time PCR is developed for the detection of chicken DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.


• Accessories: SureFood® PREP Basic |SureFood® PREP Advanced |SureFast® Mag PREP Food|TANBead Maelstrom™ 4800


• Related products: RIDA®CYCLER|SureFood® ANIMAL QUANT Chicken |SureFood® ANIMAL ID 4plex Pork/Chicken/Turkey+IAAC |SureFood® ANIMAL ID 4plex Beef/Sheep/Goat+IAAC

General Information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 Agilent Mx3005P. *note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.