SureFood® ALLERGEN 4plex EU NUTS

Intended use:

The SureFood® ALLERGEN 4plex EU NUTS is a multiplex real-time PCR for the direct, qualitative detection and differentiation of

• almond (Prunus dulcis),
• cashew (Anacardium occidentale),
• pistachio (Pistacia vera),
• peanut (Arachis hypogaea),
• hazelnut (Corylus avellana),
• walnut (Juglans regia),
• pecan (Carya illinoinensis),
• brazil nut (Bertholletia excelsa) and
• macadamia (Macadamia tetraphylla)

ccording to directive (EC) 1169/2011 in food.

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.


• LOD (Detection limit): The detection limit is = 1 mg allergenic substance / kg food (ppm) using SureFood® PREP Advanced. The LOD was determined using laboratory reference material SureFood® QUANTARD Allergen 40 (containing 40 mg/kg nuts in a corn flour matrix).


• Cross reactivity:

  The pecan detection system shows 100% cross reactivity to shagbark hickory (Carya ovata).



• Accessories: SureFood® PREP Advanced |SureFast® Mag PREP Food


• Related products: SureFood® ALLERGEN 4plex Peanut / Hazelnut / Walnut + IAC |SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC|SureFood® ALLERGEN Almond|SureFood® QUANTARD Allergen 40 |SureFood® ALLERGEN Pistachio|RIDA®CYCLER|TANBead Maelstrom™ 4800

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96Dx, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.