{"product_id":"surefast®-escherichia-coli-serotype-ii-4plex","title":"SureFast® Escherichia coli Serotype II 4plex","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nThe SureFast® Escherichia coli Serotype II 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific \u003cem\u003ewzx\u003c\/em\u003e gene DNA sequences of \u003cem\u003eEscherichia coli\u003c\/em\u003e O45, \u003cem\u003ewbdl\u003c\/em\u003e gene DNA sequences of \u003cem\u003eEscherichia coli\u003c\/em\u003e O111 and \u003cem\u003eihp1\u003c\/em\u003e gene DNA sequences of O145.\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: 100 reactions\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended. To assess the process of bacterial growth, it is suggested to compare the samples at the beginning and at the end of the culturing (bacterial growth at Cp difference \u0026gt; 3). For cultivation the method of the ISO\/TS 13136 is recommended.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): The SureFast® Escherichia coli Serotype II 4plex real-time PCR has a limit of detection of \u0026lt; 5 DNA copies.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAccessories: SureFast® Speed PREP |SureFast® PREP Bacteria|SureFast® Mag PREP Pathogens|TANBead Maelstrom™ 4800\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: SureFast® Escherichia coli Serotype I 4plex|SureFast® EHEC\/EPEC 4plex |SureFast® STEC 4plex ONE|SureFast®STEC Screening PLUS |RIDA®CYCLER|SureFast® Foodborne Pathogens 4plex\u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nThe real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC\/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Applied Biosystems 7500 and R-Biopharm RIDA®CYCLER.","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657892258083,"sku":"RBIOF5168","price":1828.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/F5168_055a989d-2a1c-4197-aa1a-524f8a6e5f2b.jpg?v=1779863335","url":"https:\/\/onelab.co.nz\/products\/surefast%c2%ae-escherichia-coli-serotype-ii-4plex","provider":"Onelab","version":"1.0","type":"link"}