SureFast® Campylobacter 4plex

Intended use:

The SureFast® Campylobacter 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Campylobacter jejuni, Campylobacter lari and Campylobacter coli.

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA-preparation the use of SureFast® PREP Bacteria (Art. No. F1021) is recommended.


• LOD (Detection limit): The SureFast® Campylobacter 4plex real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.


• Accessories: SureFast® PREP Bacteria


• Related products: SureFast® Mag PREP Pathogens|TANBead Maelstrom™ 4800|TANBead Maelstrom™ 8 Autostage|RIDA®CYCLER|SureFast® Speed PREP

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.