SureFast® Yersinia 3plex

Intended use:

The SureFast® Yersinia 3plex is a real-time PCR for the direct, qualitative detection and differentiation of specific ail gene DNA sequences of Yersinia pseudotuberculosis and Yersinia enterocolitica.

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA-preparation the use of SureFast® PREP Bacteria is recommended.


• LOD (Detection limit): The SureFast® Yersina 3plex real-time PCR has a limit of detection of < 5 DNA copies.


• Cross reactivity:

  The detection system of Yersinia pseudotuberculosis shows a cross reactivity to Yersinia pestis.



• Accessories: SureFast® PREP Bacteria|SureFast® Speed PREP |SureFast® Mag PREP Pathogens|TANBead Maelstrom™ 4800


• Related products: RIDA®CYCLER|SureFast® Vibrio 4plex |SureFast® Foodborne Pathogens 4plex

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.