SureFast® VEGAN

Intended use:

The SureFast® VEGAN real time PCR is a highly sensitive system for the detection of traces of vertebrate-DNA and differentiation between DNA of vertebrates and plants in food. The Positive Control correspondents to 0.1% vertebrate-DNA. Insects, arachnids, molluscs and crustaceans are not detected by this system.

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA-preparation the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended.


• LOD (Detection limit): The SureFast® VEGAN real-time PCR has a limit of detection of 0.01%. The limit of detection of the complete method (DNA extraction and real-time PCR) depends on sample matrix, processing grade, DNA-preparation and DNA-content.


• Accessories: SureFood® PREP Basic |SureFood® PREP Advanced |SureFast® Mag PREP Food|TANBead Maelstrom™ 4800


• Related products: RIDA®CYCLER|SureFast® Animal+Plant Control 3plex|SureFood® ALLERGEN Fish|SureFood® ALLERGEN Crustaceans|SureFood® ALLERGEN Molluscs

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96Dx, Bio-Rad CFX Opus, R-Biopharm RIDA®CYCLER, Agilent AriaDx.