SureFast® STEC 4plex ONE

Intended use:

The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli serotype O157 in different types of food. Two in ONE - STEC 4plex test kit with included DNA preparation Benefits:

• Integrated DNA preparation
• Use the DNA directly for qPCR
• Open system for all common qPCR thermocyclers

Specifications:

• Test format: 100 reactions


• Sample preparation: 10 min lysis step


• LOD (Detection limit): The SureFast® STEC 4plex ONE real-time PCR has a limit of detection of < 5 DNA copies.


• Accessories: RIDA®CYCLER


• Related products: SureFast® Salmonella ONE |SureFast® Escherichia coli Serotype I 4plex|SureFast® Escherichia coli Serotype II 4plex|SureFast® EHEC/EPEC 4plex |SureFast® Escherichia coli PLUS |SureFast®STEC Screening PLUS |SureFast® Foodborne Pathogens 4plex|SureFast® Listeria 3plex ONE

General Information:

The multiplex real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Qiagen Rotor-Gene Q and Bio Molecular Systems MIC.