SureFast® Legionella Screen PLUS

Intended use:

The test detects Legionella spp. DNA. Each reaction contains an internal amplification control (IAC).  

Specifications:

• Test format: 100 reactions


• Sample preparation: For DNA-preparation the use of SureFast® PREP Aqua is recommended.


• LOD (Detection limit): The SureFast® Legionella Screen PLUS real-time PCR has a limit of detection of =5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.


• Accessories: SureFast® PREP Aqua


• Related products: SureFast® Legionella pneumophila PLUS |SureFast® Legionella 3plex |RIDA®CYCLER

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.