Sera-Mag Oligo (dT) Coated Magnetic particles, 100ml

Overview:
Sera-Mag Oligo(dT)-Coated Magnetic Particles enable mRNA isolation and extraction for applications such as RT-PCR, cDNA library construction, cDNA microarrays, affinity purification, primer extension, and subtractive hybridization.

•Encapsulation means no exposed iron and minimized interference with downstream enzymatic applications
•Covalently bound oligo(dT)14 prevents leaching from the particle surface
•Very high, specific poly A+ binding capacity ensures maximum extraction of mRNA
•Fast reaction kinetics increase throughput and precision, also enabling faster movement through viscous solutions
•Uniform, nominal 1 µm diameter provides high surface area and excellent lot-to-lot reproducibility
•Also available as custom-manufactured magnetic beads

Convenient and efficient mRNA isolation:
Eukaryotic mRNA makes up only 1 to 3% of total cellular RNA, so efficient mRNA purification is critical. Sera-Mag Oligo(dT) Magnetic Particles remove 90% or more of mRNA from total RNA with just one extraction. Sera-Mag Oligo(dT) Magnetic Particles bind target mRNA through pairing of the polyadenylated RNA tail found on the 3’ end of mRNA to the covalently bound oligo(dT) groups on the surface of the particles. This binding is easily accomplished using standard hybridization conditions during the mRNA purification process.

Fast magnetic response times and stable physical integrity:
The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One layer of magnetite is coated onto this core, while the surface is modified to minimize non-specific binding of proteins. Oligo(dT) groups are then covalently bound to the particle surface. Since the magnetite is encapsulated, there is no exposed iron minimizing interference with downstream enzymatic applications.