{"product_id":"ridascreen-elisa-gliadin-96-determinations","title":"RIDASCREEN ELISA Gliadin , 96 determinations","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nRIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food.\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\nIt is applicable for the quantitative measurement of intact gliadin as a measure of gluten in unprocessed and processed matrices from important gluten-free food categories, including rice- and corn-based products, soy, starches, pseudo cereals, legumes, spices, juice, nut nougat crème, cream cheese, pesto, meat, vegetarian meat alternative, cookies, dessert, cake, fish, bread, candies, and potatoes. The sandwich ELISA quantifies intact gliadin from wheat and related proteins from rye and barley. This method is not accurate for quantification of fermented or hydrolyzed gluten.\u003cbr\u003e\r\n\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: Homogenization and extraction\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eIncubation time: 1 h 30 min, Room temperature\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): \u003cbr\u003e\r\n-0.5 mg \/ kg (ppm) Gliadin (mean) or 1 mg \/ kg (ppm) Gluten \u003cbr\u003e\r\n-0.06 - 1.24 mg \/ kg (ppm) Gliadin* (*depending on matrix) \u003cbr\u003e\n\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOQ (Limit of quantification): 2.5 mg \/ kg (ppm) Gliadin or 5 mg \/ kg (ppm) Gluten\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eValidated matrices: Rice- and corn-based products, soy, starches, pseudo cereals, legumes, spices, juice, nut nougat crème, cream cheese, pesto, meat, vegetarian meat alternative, cookies, dessert, cake, fish, bread, candies, and potatoes\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAvailable application notes: \u003cul\u003e\r\n\r\n  \u003cli\u003eAllergen swabbing using RIDASCREEN® Allergen ELISAs.\u003c\/li\u003e\r\n\r\n  \u003cli\u003eRIDASCREEN Gliadin in combination with RIDA® Extraction Solution (colorless) (R7098) – only after validation.\u003c\/li\u003e\r\n\r\n  \u003cli\u003eSample preparation for raw materials with ethanol.\u003c\/li\u003e\r\n\r\n  \u003cli\u003eSample preparation for polyphenol containing raw materials (e.g. chocolate, cofee, cacao, buckwheat) with fish gelatine and ethanol.\u003c\/li\u003e\r\n\r\n\u003c\/ul\u003e\n\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eDetected analyte: QQPFP epitope that occurs in a-\/B-, y-, and ?-gliadins; B-, C-, and y-hordeins; as well as y- and ?-secalins from wheat, rye, and barley\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eEvaluation: Microtiter plate spectrophotometer (450 nm)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAccessories: Cocktail (patented) (en)|Cocktail ECO|RIDA® Extraction Solution (colorless) (en)|Set of 3 processed Gliadin Assay Controls (en)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: RIDASCREEN® Total Gluten|RIDA®QUICK Gliadin (en)|SureFood® ALLERGEN Gluten\u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\u003cbr\u003e\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nGluten is a mixture of prolamin and glutelin proteins present in wheat, rye and barley. Prolamins from wheat are named gliadins.\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe use of gluten in foodstuffs is extremely common because of its useful effects on e.g. texture, moisture retention and flavor. However, gluten intolerance disorders like coeliac disease require a permanent gluten-free diet to avoid clinical symptoms. According to the Codex Alimentarius (CODEX STAN 118\/1979) two categories for labelling of dietary food according to the gluten content exist:\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\n\u003cp style=\"padding-left: 40px\"\u003e1) \"Glutenfree\" = Food products, containing \u0026lt; 20 mg \/ kg gluten\r\n\u003cbr\u003e\r\n2) \"Very low gluten\" = Food products containing 20 - 100 mg \/ kg gluten\u003c\/p\u003e\r\n\u003cbr\u003e\r\nThe threshold of 20 mg \/ kg has been adopted by many national legislations in many countries. The Méndez method – a combination of R5 ELISA and a special, patented buffer (cocktail) for optimized extraction of gliadin from heat-processed and non-heated food samples – is the Codex Alimentarius recommended method for analysis of gluten in food. It is Codex Alimentarius type I method for analysis of gliadin\/gluten. Categorization as type I method defines it as reference method. R-Biopharm is the only R5 ELISA manufacturer who offers the licensed Cocktail (patented) (Art. No. R7006 \/ R7016) and hence fulfills the requirements of the reference method to 100 %.\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\n\r\nFurthermore, RIDASCREEN® Gliadin is in combination with the Cocktail (patented) the only R5 ELISA, which is AOAC Official Method of Analysis (OMA 2012.01). Applicability for analysis of principally all foods is an important requirement to function as Méndez method in the meaning of Codex Alimentarius. The publication of the 3rd  international collaborative proficiency study from 2020 describes the preparation of 900 samples of 19 differently processed foods from 16 food categories and the obtained results demonstrating it suitability for gluten detection in gluten-free foods. Accordingly, RIDASCREEN® Gliadin received from the AOAC as first assay worldwide the status as Official Method of Analysis (AOAC OMA 2012.01) “in food” in 2021. A limitation on certain validated food matrices as usual for other AOAC approvals is not present any longer.\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\n\r\n\u003cstrong\u003eFurther approvals:\u003c\/strong\u003e\r\n\r\nThe RIDASCREEN® Gliadin in combination with the Cocktail (patented) is not only Codex Alimentarius type I method and AOAC Official Method of Analysis. It is also revised and approved method of AOAC-RI, AACCI and ICC (see below “Certificates”).\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\n\r\n\u003cem\u003ePatent Marking:\u003c\/em\u003e\r\n\u003cbr\u003e\r\n\u003cem\u003eIf the R-Biopharm product “Cocktail ECO” is used for the processing of food samples, a sulphite-containing extraction agent is used. Food inspection methods using a sulfite-containing extractant as in this product and\/or corresponding detection kits are subject to the following patents of MORINAGA \u0026amp; Co., Ltd.: European Patent EP 2 224 239 B1, Australian Patent AU 2008 330 507 B2, United States Patent US 8 859 212 B2, Japanese Patent JP 5 451 854 B2. The patent holder has granted R-Biopharm AG a license to use, and sell products that employ, said protected technology in the above-mentioned territories.\u003c\/em\u003e","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":48119535698211,"sku":"RBIOR7001","price":636.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/R7001.jpg?v=1787150133","url":"https:\/\/onelab.co.nz\/products\/ridascreen-elisa-gliadin-96-determinations","provider":"Onelab","version":"1.0","type":"link"}