RIDASCREEN®FAST ß-Lactoglobulin

Intended use:

RIDASCREEN®FAST ß-Lactoglobulin is a sandwich enzyme mmunoassay for the quantitative analysis of native and processed ß-lactoglobulin in rice crispies, chocolate and sausage.

Specifications:

• Test format: Microtiter plate with 48 wells (6 strips with 8 removable wells each)


• Sample preparation: Homogenization and extraction


• Incubation time: 30 min Room temperature


• LOD (Detection limit): 0.042 mg / kg (ppm) ß-lactoglobulin (mean) 0.024 – 0.073 mg / kg (ppm) ß-lactoglobulin* *depending on matrix



• LOQ (Limit of quantification): 0.167 mg / kg (ppm) ß-lactoglobulin


• Cross reactivity:

  There is a cross reactivity to sheep’s, goat’s and buffalo’s milk.



• Validated matrices: Rice crispies, chocolate and sausage.


• Available application notes:
  • Extraction for heavily liquid absorbing matrices using RIDA® Extractor 2 (R4613).
  • Sample extraction of pine nuts, sunflower and pumpkin seed.


• Detected analyte: ß-lactoglobulin of cow’s milk


• Evaluation: Microtiter plate spectrophotometer (450 nm)


• Related products: RIDASCREEN®FAST Milk |RIDASCREEN®FAST Casein |RIDASCREEN® ß-Lactoglobulin

General Information:

Cow’s milk contains 3.2 % proteins which consist of 10 % ß-lactoglobulin (leading protein of whey) and 80 % caseins. The most important allergen especially for children is ß-lactoglobulin. For adults the caseins become more dominant. Milk can induce allergic reactions at infancy. Whey or milk powder is often added to food products, therefore it is recommended to determine ß-lactoglobulin in food. The allergen can be present as an ingredient or as a contamination in raw and cooked products. According to the regulation (EU) No. 1169/2011, milk and products thereof must be declared on food labels. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand.

Benefits:

• Fast: Only 30 minutes incubation time during procedure (by far the fastest compared to competitors).
• Specificity: no cross reactivities have been observed in a wide range of matrices (85).
• Validated for automates.