{"product_id":"ridascreen®fast-gliadin-en","title":"RIDASCREEN®FAST Gliadin","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nRIDASCREEN® FAST Gliadin is a sandwich enzyme immunoassay for the quantitative analysis of contaminations by prolamins from wheat (gliadin), rye (secalin), and barley (hordein) in raw products like flours (buckwheat, rice, corn, oats, teff) and spices as well as in processed foods like noodles, ready-to-serve meals, bakery products, sausages, beverages and ice cream.\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: Microtiter plate with 48 wells (6 strips with 8 removable wells each)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: Homogenization and extraction\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eIncubation time: 30 min\r\nRoom temperature\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): ''- Cocktail (patented) 0.50 mg \/ kg (ppm) Gliadin (mean) or to 1.0 mg \/ kg (ppm) Gluten\r\n- Cocktail ECO 0.52 mg \/ kg (ppm) Gliadin (mean) or to 1.04 mg \/ kg (ppm) Gluten\r\n(depending on matrix)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOQ (Limit of quantification): 5 mg \/ kg (ppm) Gliadin or 10 mg \/ kg (ppm) Gluten\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eValidated matrices: Contaminations by prolamins from wheat (gliadin), rye (secalin), and barley (hordein) in raw products like flours (buckwheat, rice, corn, oats, teff) and spices as well as in processed food like noodles, ready-to-serve meals, bakery products, sausages, beverages and ice cream.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAvailable application notes: \u003cul\u003e\r\n \t\u003cli\u003eSample preparation for processed food with the RIDA® Extraction Solution (colorless) (R7098) – only after validation.\u003c\/li\u003e\r\n \t\u003cli\u003eSample preparation for raw materials with ethanol.\u003c\/li\u003e\r\n \t\u003cli\u003eSample preparation for polyphenol containing raw materials (e.g. chocolate, cofee, cacao, buckwheat) with fish gelatine and ethanol.\u003c\/li\u003e\r\n\u003c\/ul\u003e\n\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eDetected analyte: \u003cul\u003e\r\n \t\u003cli\u003eProlamins from wheat, rye and barley (spelt, kamut)\u003c\/li\u003e\r\n \t\u003cli\u003eEpitopes which are related to celiac disease (beside others one peptide sequence in the a-gliadin)\u003c\/li\u003e\r\n \t\u003cli\u003e?-gliadin\u003c\/li\u003e\r\n\u003c\/ul\u003e\n\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eEvaluation: Microtiter plate spectrophotometer (450 nm)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: RIDASCREEN® Gliadin \u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nThe use of wheat flour and gluten in foodstuffs is extremely common because of their heat stability and useful effects on e.g. texture, moisture retention and flavour. Gluten is a mixture of prolamin and glutelin proteins present in wheat, rye and barley. Coeliac disease is a permanent intolerance to gluten that results in damage to the small intestine and is reversible when gluten is avoided by diet. According to the Codex Alimentarius (CODEX STAN 118\/1979) two categories for labeling of food according to the gluten content now exist:\r\n1.) “Glutenfree” = Food products, containing \u0026lt; 20 mg \/ kg Gluten\r\n2.) “Very low gluten” = Food products containing 20 – 100 mg \/ kg Gluten\r\nThe threshold of 20 mg \/ kg has been adopted by many national legislations in many countries.\r\n\r\nThe sample preparation using the Cocktail (patented) (R7006 \/ R7016) is the official R5-Mendez method according to the Codex Alimentarius and the AOAC. The faster sample preparation using the environmental-friendly Cocktail ECO (R7080) is convenient for the screening of samples. The Cocktail ECO has an extraction efficiency of approx. 70 - 110 % compared to Cocktail (patented).","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657893634339,"sku":"RBIOR7002","price":605.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/R7002_4d7f58c6-e7aa-4796-a8da-a1706324c748.jpg?v=1779863409","url":"https:\/\/onelab.co.nz\/products\/ridascreen%c2%aefast-gliadin-en","provider":"Onelab","version":"1.0","type":"link"}