{"product_id":"ridascreen®-set-a-b-c-d-e-en","title":"RIDASCREEN® SET A,B,C,D,E","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nRIDASCREEN® SET A,B,C,D,E is a sandwich enzyme immunoassay for the identification of \u003cstrong\u003eStaphylococcus enterotoxins A, B, C, D and E \u003c\/strong\u003ein fluid and solid foods as well as in bacterial cultures. Based on its sensitivity the RIDASCREEN® SET A,B,C,D,E test is consequently clear superior to the immunodiffusion procedure which has a detection limit of 0.1 mg \/ ml.\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: Microtiter plate with 96 wells (12 strips with 8 wells each),\r\none strip is necessary for analysis of one sample,\r\n12 tests in total.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: „Official European Preparation Method“ (dialysis concentration method) or\u003cbr\u003e\u003cbr\u003e\r\nsimplified method.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eIncubation time: 2 h 45 min\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): Simplified working up:\r\n-  Liquid samples: 0.25 ng toxin \/ ml sample,\r\n-  Solid samples: 0.375 ng toxin \/ g sample,\r\n-  Supernatants from bacterial cultures: 0.25 ng toxin \/ ml sample.\r\n\r\nDialysis concentration:\r\n-  Liquid samples: 0.05 ng toxin \/ ml sample,\r\n-  Solid samples: 0.05 ng toxin \/ g sample.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eCross reactivity: \u003cp\u003eCrossreactivities are known to occur between antibody \/ toxin:\u003cbr\u003e\r\nA\/E, E\/A, B\/C and C\/B.\u003c\/p\u003e\r\n\u003cp\u003eThe percentage of crossreactivities is between 10 – 20 %. Some food matrices (e.g.: milk, sausages) may enhance or weaken the crossreactivity.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eDetected analyte: Staphylococcus enterotoxins A, B, C, D, E\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eEvaluation: Microtiter plate spectrophotometer\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nStaphylococci belong to the family of micrococci. \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, \u003cem\u003eStaphylococcus hyicus\u003c\/em\u003e as well as \u003cem\u003eStaphylococcus intermedius\u003c\/em\u003e produce one or more heat stable proteins which behave as enterotoxins (SET). SETs are the main causative agents of food poisoning next to Salmonellosis and Campylobacteriosis. Generally, it is assumed that a population of 5 x \u003cspan class=\"st\"\u003e\u003cem\u003e10\u003c\/em\u003e\u003csup\u003e5\u003c\/sup\u003e\u003c\/span\u003e cells of enterotoxin-producing \u003cem\u003eS. aureus\u003c\/em\u003e strains per gram of food is required to lead to an intoxication. However, other studies show that only 100 - 200 ng of Staphylococcus enterotoxins can lead to symptoms of food poisoning. SET intoxications have been frequently associated with pasta, finished meat products, ham, pies, chicken meat products, fish, fish products, milk, milk products, ice-cream, egg products, salads, pastries and cake stuffing as well as preparations from these food products. The enterotoxins of the serological group A, B, C, D, E are very significant.","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657899893027,"sku":"RBIOR4101","price":440.4,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/R4101_f2edfa84-d3e3-4ba0-b3f2-9f32dda8af55.jpg?v=1779863643","url":"https:\/\/onelab.co.nz\/products\/ridascreen%c2%ae-set-a-b-c-d-e-en","provider":"Onelab","version":"1.0","type":"link"}