RIDASCREEN® DNSH

Intended use:

RIDASCREEN® DNSH (Art. No. R3740) is a competitive enzyme immunoassay for the quantitative analysis of nifursol metabolite 3,5-dinitrosalicylic acid hydrazide (DNSH) in meat (poultry, pork, beef) and seafood (fish, shrimp, and prawn).

Specifications:

• Test format: Microtiter plate with 96 wells (12 strips with 8 wells each)


• Sample preparation: Homogenization and extraction


• Incubation time: 1 h (20 - 25 °C)


• LOD (Detection limit): Meat approx. 0.13 µk/kg (ppb), fish and shellfish approx. 0.15 µg/kg


• Detection capability CCB: 0.25 µg/kg


• Validated matrices: Meat, fish and shellfish.


• Detected analyte: 3,5-Dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.


• Evaluation: Microtiter plate spectrophotometer (450 nm)


• Related products: RIDASCREEN® Nitrofuran (AHD) |RIDASCREEN® Nitrofuran (AMOZ) |RIDASCREEN® Nitrofuran (SEM)

General Information:

Nifursol is a nitrofuran antibiotic banned as a feed additive in the European Union and other countries. Nifursol is metabolized to 3,5-dinitrosalicylic acid hydrazide (DNSH) in living organisms. DNSH is a marker for the detection of illegal use of nifursol in animal husbandry. The detection of DNSH by LC-MS/MS requires derivatization of this metabolite with 2-nitrobenzaldehyde to NP-DNSH, like other nitrofuran metabolites such as SEM, AHD, AMOZ and AOZ. The antibody used in this test detects DNSH without derivatization with 2-nitrobenzaldehyde. In accordance with Commission Regulation (EU) 2019/1871 a reference point for action (RPA) of 0.5 µg/kg for DNSH and other nitrofuran metabolites applies from 28 November 2022.