KOBRA CELL®

Intended use:

Electrochemical cell for the derivatisation of aflatoxins B1 and G1 using HPLC.

General Information:

The KOBRA® CELL is based on an electrochemical cell, fitted between the HPLC column, the detector which consists of a platinum working electrode and a stainless steel auxiliary electrode, separated by an ion exchange membrane. The principle of the technique is to deliver to the KOBRA® CELL, the mobile phase containing the derivatisation agent precursor, a potassium bromide salt, and aflatoxins. Bromine is then generated electrochemically by applying a constant potential at the working electrode. The resulting electrochemical reaction enables aflatoxins B1 and G1 to be derivatised in-situ to their brominated derivatives, which gives an enhanced fluorescence response. A KOBRA® CELL measures 10 x 10 x 5 cm.

Specifications:

• Test format: Each pack contains: 1 KOBRA® CELL (size 10 x 10 x 5 cm), 1 Power Pack, 2 Electrode Connection Leads, 50 cm x 0.5 mm ID PEEK Tubing, 1 Spare Membrane


• Sample preparation:


• Incubation time:


• LOD (Detection limit): Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used. B1 0.031 ng/ml B2 0.016 ng/ml G1 0.031 ng/ml G2 0.004 ng/ml


• Approvals: Listed in CEN and AOAC Official Methods as a recognised electrochemical derivatisation system


• Accessories: KOBRA® CELL Installation Pack (en)|KOBRA® CELL Membrane (en)