HisTrap column FF 5 X 5ml

Prepacked Ni2+ Sepharose columns for histidine-tagged protein purification

•High binding capacity, approx. 40 mg/mL resin.
•Negligible leakage of Ni2+.
•Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
•Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.


Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications