{"product_id":"hisprep-column-ff-16-10","title":"HisPrep column FF 16\/10","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nHisPrep FF 16\/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Optimized for convenient scale-up purification of histidine-tagged proteins.\u003cbr\u003e\r\n•Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.\u003cbr\u003e\r\n•Negligible leakage of Ni2+.\u003cbr\u003e\r\n•High binding capacity, approx. 40 mg\/mL resin.\u003cbr\u003e\r\n•Convenient and time-saving 20 mL prepacked HiPrep format.\u003cbr\u003e\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\nHisPrep FF 16\/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.\u003cbr\u003e\r\n\u003cbr\u003e\r\n*Image is a sample only*\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":53660018802979,"sku":"CYTI28936551","price":1190.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/28936551.jpg?v=1788228019","url":"https:\/\/onelab.co.nz\/products\/hisprep-column-ff-16-10","provider":"Onelab","version":"1.0","type":"link"}