HisPrep column FF 16/10

Overview
HisPrep FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

•Optimized for convenient scale-up purification of histidine-tagged proteins.
•Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
•Negligible leakage of Ni2+.
•High binding capacity, approx. 40 mg/mL resin.
•Convenient and time-saving 20 mL prepacked HiPrep format.


HisPrep FF 16/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

*Image is a sample only*