EuroProxima Ochratoxin A

Intended use:

EuroProxima Ochratoxin A is a competitive enzyme immunoassay for quantitative analysis of ochratoxin A in corn, wheat, red wine, white wine, must, roasted coffee, instant coffee, green coffee, cocoa, figs and raisins.

Specifications:

• Test format: Microtiter plate with 96 wells (12 strips with 8 wells each).


• Sample preparation: For mentioned matrices fast and efficient extraction methods are included in the kit description.


• Incubation time: 1 h 30 min


• LOD (Detection limit): Corn (1.4 µg/kg (ppb)), wheat (1.7 µg/kg), red wine (0.3 µg/L), white wine (0.3 µg/L), must (0.3 µg/kg), roasted coffee (1.9 µg/kg), instant coffee (1.8 µg/kg), green coffee (1.2 µg/kg), cocoa (1.7 µg/kg), figs (0.7 µg/kg), raisins (3.2 µg/kg)


• Validated matrices: Corn, wheat, red wine, white wine, must, roasted coffee, instant coffee, green coffee, cocoa, figs and raisins.


• Detected analyte: Ochratoxin A in corn, wheat, red wine, white wine, must, roasted coffee, instant coffee, green coffee, cocoa, figs and raisins.


• Evaluation: Microtiter plate spectrophotometer (450 nm)

General Information:

Ochratoxin A is a nephrotoxic and nephrocarcinogenic mycotoxin produced by Penicillium verrucosum and Penicillium viridicatum in temperate and cold climates and by a number of Aspergillus species such as A. ochraceus in warmer and tropical areas of the world. Ochratoxin A has been shown to occur in various cereals and other plant products, coffee beans and coffee products, wine, and feed. In the European Union maximum levels (MLs) for ochratoxin A have been set for different food commodities. The MLs vary from 0.5 to 20 µg/kg (ppb) depending on the food type. The guidance limits for feed are 250 µg/kg for cereals and cereal products and 50 and 100 µg/kg for complete and complementary feeding stuffs for pigs and poultry, respectively.