EuroProxima Chloramphenicol Fast

Intended use:

EuroProxima Chloramphenicol Fast is a competitive enzyme immunoassay for quantitative analysis of chloramphenicol in urine (direct), urine (extraction), liver, tissue, milk, milk (direct), feed, egg, honey and serum.

Specifications:

• Test format: Microtiter plate with 96 wells (12 strips with 8 wells each).


• Sample preparation: For mentioned matrices fast and efficient extraction methods are included in the kit description.


• Incubation time: 45 min


• LOD (Detection limit): Urine (direct) (0.5 µg/L (ppb)), urine (extraction) (0.02 µg/L), liver (0.02 µg/kg), tissue (0.02 µg/kg), milk (0.02 µg/L), milk (direct) (0.2 µg/L), feed (0.5 µg/kg), egg (0.02 µg/kg), honey (0.02 µg/kg), serum (0.2 µg/L)


• Validated matrices: Urine (direct), urine (extraction), liver, tissue, milk, milk (direct), feed, egg, honey and serum.


• Detected analyte: Chloramphenicol in urine (direct), urine (extraction), liver, tissue, milk, milk (direct), feed, egg, honey and serum.


• Evaluation: Microtiter plate spectrophotometer (450 nm)

General Information:

The use of the broad-spectrum antibiotic chloramphenicol (CAP) was totally banned within the European Union by modification of Annex IV of council Regulation (EEC) 2377/90 with Commission Regulation 1430/94. In 2003, a minimum required performance limit (MRPL) for CAP residues in food of animal origin was set at 0.3 µg/kg. This CAP-ELISA is suitable for the detection of GAP and its glucuronide (major metabolite urine). CAP can be detected in urine directly (after a dilution in buffer), or after ethyl acetate extraction for increased sensitivity and specificity. Defatted milk samples can also be applied to the test directly with a detection limit of 0.1 ng/ml. This detection limit can be lowered ten times using an extraction in ethyl acetate. For eggs and tissue samples, including shrimps, crab and fish, the ethyl acetate extraction is followed by a clean-up step using a mixture of iso-octane/ trichloromethane or n-hexane resulting in detection limits down to levels of 0.04- 0.02 ng/g.