EuroProxima Bacitracin

Intended use:

EuroProxima Bacitracin is a competitive enzyme immunoassay for quantitative analysis of Bacitracin in urine, tissue, milk, feed and egg.

Specifications:

• Test format: Microtiter plate with 96 wells (12 strips with 8 wells each).


• Sample preparation: For mentioned matrices fast and efficient extraction methods are included in the kit description.


• Incubation time: 1 h 30 min


• LOD (Detection limit): Urine (23 µg/L (ppb)), tissue (9 µg/kg), milk (10 µg/L), feed (60 µg/kg), egg (11 µg/kg)


• Validated matrices: Urine, tissue, milk, feed and egg.


• Detected analyte: Bacitracin in urine, tissue, milk, feed and egg.


• Evaluation: Microtiter plate spectrophotometer (450 nm)

General Information:

Antibacterial drugs intended to be used as growth promoters are added to the feed of entire herds and flocks at sub-therapeutic levels over an extended period of time. These performance enhancing antibacterial growth promoters are regulated by Council Directive 70/524/EEC as zootechnical feed additives and specified tolerances for their inclusion are given in the annex of that document. Whenever drug preparations are administered to food-producing animals, residues thereof in edible tissues, milk or eggs are likely. Residues of antibacterial drugs in food could lead to allergic reactions but the greatest threat is the development of resistant strains of bacteria which could lead to an improper response to normal drug treatment in humans. For these reasons the European Commission decided to ban some of the regulated growth promoters. With Council Regulation 2821/98 zinc bacitracin, spiramycin, tylosin and virginiamycin, and with Commission Regulation 2788/98 olaquindox, were banned from animal feed.