{"product_id":"easi-extract-vitamin-b12-lge-rbrp88b-50-immunoaffinity-columns-with-10ml-format","title":"EASI-EXTRACT Vitamin B12 (LGE) (RBRP88B=50 immunoaffinity columns with 10ml format)","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nImmunoaffinity columns for use in conjunction with an HPLC or LC-MS\/MS system for detection of vitamin B12 in a wide range of commodities.\r\n\u003cbr\u003e\r\n\r\n\r\nNovel immunoaffinity columns with built-in reservoir for detection of vitamin B12 in a wide of range commodities, developed in conjunction with Nestlé Research Centre, Switzerland.\u003cbr\u003e\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: \u003cbr\u003e\r\n-RBRP88 = 10 immunoaffinity columns with 10 ml format.\u003cbr\u003e\r\n-RBRP88B = 50 immunoaffinity columns with 10 ml format.\u003cbr\u003e\n\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: Sample is extracted using buffer and enzymes, filtered and diluted ready for applying to the immunoaffinity column. The LGE column is a unique format with no other immunoaffinity column having this design. This immunoaffinity allows the sample extract to mix directly with the antibody gel within the column ensuring that the best recoveries and lowest % RSD are achieved.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eIncubation time: Sample is incubated for 30 mins at 37 °C followed by 30 mins at 100 °C.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eLOD (Detection limit): Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependent on method, LC conditions and equipment used.  \r\nCyanocobalamin 5 ng \/ ml\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAvailable application notes: Methods are available for a range of commodities. Please contact your local R-Biopharm distributor for further information.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eDetected analyte: Cyanocobalamin\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eEvaluation: HPLC or LC-MS\/MS system\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eApprovals: Product referenced in AOAC Official Final Action Method 2014.02\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eAccessories: Immunoaffinity Column Rack  (en)|Immunoaffinity Column Accessory Pack (en)\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: EASI-EXTRACT® VITAMIN B12 (en)\u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nThe procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Following extraction of the vitamin the sample extract is diluted with buffer and filtered before being passed slowly through the immunoaffinity column. Any vitamin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS\/MS.\r\n\u003cbr\u003e\r\n\r\n\u003ch3\u003eBenefits:\u003c\/h3\u003e\r\n\r\n\u003cul\u003e\r\n\r\n  \u003cli\u003eAOAC Official Final Action method 2014.02.\u003c\/li\u003e\r\n\r\n  \u003cli\u003e\r\n\r\n\u003cdiv\u003eUnique format immunoaffinity column.\u003c\/div\u003e\n\u003c\/li\u003e\r\n\r\n  \u003cli\u003eUses monoclonal antibody.\u003c\/li\u003e\r\n\r\n  \u003cli\u003eExtraction uses buffer and enzymes.\u003c\/li\u003e\r\n\r\n  \u003cli\u003eCan be used with pigmented samples.\u003c\/li\u003e\r\n\r\n  \u003cli\u003eCan be stored at 21 - 25 °C.\u003c\/li\u003e\r\n\r\n  \u003cli\u003eThe columns are suitable for testing a wide range of commodities.\u003c\/li\u003e\r\n\r\n\u003c\/ul\u003e","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":48119538352419,"sku":"RBIORBRP88B","price":2001.03,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/RBRP88andRBRP88B.jpg?v=1794317724","url":"https:\/\/onelab.co.nz\/products\/easi-extract-vitamin-b12-lge-rbrp88b-50-immunoaffinity-columns-with-10ml-format","provider":"Onelab","version":"1.0","type":"link"}