{"title":"PCR clean up \u0026 size selection","description":"","products":[{"product_id":"amersham-microspin-s-300-hr-columns","title":"Amersham MicroSpin S-300 HR Columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nMicroSpin S-300 HR spin columns for DNA purification from unincorporated primers (\u0026lt;20-mers) and nucleotides using spin-column chromatography in applications including desalting, buffer exchange, and primer removal.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAccommodates 25 to 50 µL: for post-PCR cleanup prior to sequencing\u003cbr\u003e\r\n•Useful for purification: for example, alkaline-denatured plasmid DNA prior to sequencing\u003cbr\u003e\r\n•Convenient: prepacked with Sephacryl™ S-300 HR pre-equilibrated in TE (Tris\/EDTA) buffer\u003cbr\u003e\r\n•Ready to use: requires less than 4 minutes from sample application through to collection of purified product\u003cbr\u003e\r\n•Tested in assays: nickase, single- and double-stranded exonuclease, and RNase\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin columns are designed for rapid nucleic acid purification for use in a wide range of applications, including desalting, buffer exchange and primer removal. Good product yield and purity are obtained with sample volumes from 25-100 µL, and from nanogram to milligram quantities of DNA.\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin S-200, S-300 and S-400 HR spin columns contain Sephacryl resin of differing pore sizes. They allow DNA purification via gel filtration. Molecules larger than the largest pores in the Sephacryl are excluded from the gel and elute first. Intermediate size molecules penetrate the matrix to varying extents, depending on their size and the resin used. Penetration of the matrix retards progress through the spin column; very small molecules elute last. The volume required to elute these small molecules is dependent on the volume available both inside and outside the pores, i.e. the bed volume.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775944839459,"sku":"CYTI27513001","price":367.8,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27513001.jpg?v=1789940716"},{"product_id":"amersham-microspin-s-400-hr-columns","title":"Amersham MicroSpin S-400 HR Columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nMicroSpin S-300 HR spin columns for DNA purification from unincorporated primers (\u0026lt;20-mers) and nucleotides using spin-column chromatography in applications including desalting, buffer exchange, and primer removal.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Accommodates 25 to 50 µL: for post-PCR cleanup prior to sequencing\u003cbr\u003e\r\n•Useful for purification: for example, alkaline-denatured plasmid DNA prior to sequencing\u003cbr\u003e\r\n•Convenient: prepacked with Sephacryl™ S-300 HR pre-equilibrated in TE (Tris\/EDTA) buffer\u003cbr\u003e\r\n•Ready to use: requires less than 4 minutes from sample application through to collection of purified product\u003cbr\u003e\r\n•Tested in assays: nickase, single- and double-stranded exonuclease, and RNase\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin columns are designed for rapid nucleic acid purification for use in a wide range of applications, including desalting, buffer exchange and primer removal. Good product yield and purity are obtained with sample volumes from 25-100 µL, and from nanogram to milligram quantities of DNA.\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin S-200, S-300 and S-400 HR spin columns contain Sephacryl resin of differing pore sizes. They allow DNA purification via gel filtration. Molecules larger than the largest pores in the Sephacryl are excluded from the gel and elute first. Intermediate size molecules penetrate the matrix to varying extents, depending on their size and the resin used. Penetration of the matrix retards progress through the spin column; very small molecules elute last. The volume required to elute these small molecules is dependent on the volume available both inside and outside the pores, i.e. the bed volume.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775944937763,"sku":"CYTI27514001","price":391.3,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27514001.jpg?v=1789940718"},{"product_id":"autoseq-g-50-250-columns","title":"AutoSeq G-50, 250 columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nAutoSeq G-50 columns are specifically designed to remove fluorescent dye-terminators from cycle sequencing reactions prior to analysis on automated sequencers.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated DNA sequencing\u003cbr\u003e\r\n•Columns are prepacked with Sephadex G-50 DNA Grade F\u003cbr\u003e\r\n•Columns are pre-equilibrated in double-distilled water with 0.05% Kathon CG\/ICP Biocide added as a preservative\u003cbr\u003e\r\n•Ready-to-use columns enable sample application through to collection of purified product in less than 4 min\u003cbr\u003e\r\n•4 minutes\u003cbr\u003e\r\n\u003cbr\u003e\r\nEffective DNA purification is essential for high-quality results on automated DNA sequencing since residual dye-terminators can obscure data.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50 DNA Grade F, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artefacts on salt-sensitive automated DNA sequencers.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775945101603,"sku":"CYTI27534002","price":1247.9,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27534003.jpg?v=1789940724"},{"product_id":"exoprostar-5000-reactions","title":"ExoProStar, 5000 reactions (US78225)","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides.\u003cbr\u003e\r\n•Enzymes provided in two separate tubes; just two simple pipetting steps are needed to prepare the reaction.\u003cbr\u003e\r\n•Fast 30 min protocol.\u003cbr\u003e\r\n•Scalable for different reaction sizes.\u003cbr\u003e\r\n•No loss of PCR product.\u003cbr\u003e\r\n•Easy to automate.\u003cbr\u003e\r\n•Complete heat inactivation of the enzymes within 15 min.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. ExoProStar improves digestion efficiency with no degradation of the target PCR product. Your sample is completely intact and ready for immediate use in manual or automated processes.\u003cbr\u003e\r\n\u003cbr\u003e\r\nFor the convenience of a single, stable enzyme mix with just one pipetting step we also offer the ExoProStar 1-Step (not sold in the USA, Canada or Japan).\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775945298211,"sku":"CYTI29002101","price":3674.1,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/29002090_9494bc2f-3d37-47cc-9d7a-170a8a91b0af.jpg?v=1789945514"},{"product_id":"amersham-microspin-s-200-hr-columns","title":"Amersham MicroSpin S-200 HR Columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nMicroSpin S-200 HR spin columns for rapid DNA purification of labeled single-stranded or double-stranded DNA fragments =50 bases in length in applications including desalting, buffer exchange, and primer removal using spin-column chromatography.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Prepacked with Sephacryl™ S-200 HR resin pre-equilibrated in TE (Tris\/EDTA) buffer\u003cbr\u003e\r\n•\u0026gt;80% sample recovery\u003cbr\u003e\r\n•Tested in nickase, single- and double-stranded exonuclease, and RNase assays\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin columns are designed for rapid nucleic acid purification for use in a wide range of applications, including desalting, buffer exchange and removal of primers. Good product yield and purity are obtained with sample volumes from 25-100 µL, and from nanogram to milligram quantities of DNA.\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin S-200, S-300 and S-400 HR spin columns contain Sephacryl resin of differing pore sizes. They allow DNA purification by the process of gel filtration. Molecules larger than the largest pores in the Sephacryl are excluded from the gel and elute first. Intermediate size molecules penetrate the matrix to varying extents, depending on their size and the resin used. Penetration of the matrix retards progress through the column; very small molecules elute last. The volume required to elute these small molecules is dependent on the volume available both inside and outside the pores i.e. the bed volume.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775945429283,"sku":"CYTI27512001","price":567.5,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27512001.jpg?v=1789940729"},{"product_id":"amersham-nick-columns","title":"Amersham NICK Columns","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\n\u003cbr\u003e\r\nNICK Columns are used for the purification of nick-translated DNA fragments and for separation of any labeled probe from unincorporated labeled nucleotides.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid purification of labeled DNA (= 20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography.\u003cbr\u003e\r\n•Prepacked with Sephadex G-50 DNA Grade (see Sephadex G-50 DNA Grade and Sephadex G-100 DNA Grade) in distilled water with 0.15% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Can accommodate sample volumes up to 100 µl\u003cbr\u003e\r\n\u003cbr\u003e\r\nNICK columns are ready for immediate use and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA molecules and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered and can then be used in DNA hybridization protocols. Maximum sample volume is 100 µL.\u003cbr\u003e\r\n\u003cbr\u003e\r\nNick translation is a technique in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques. It can also be used for radioactive labeling in techniques such as Southern blotting.\u003cbr\u003e\r\n\u003cbr\u003e\r\nThis process is called nick translation because the DNA to be labelled is treated with DNase to produce single-stranded \"nicks.\" This is followed by replacement in nicked sites by DNA polymerase I, which elongates the 3' end, removing nucleotides by 5'-3' exonuclease activity, and replacing them with dNTPs. To label a DNA fragment for use as a probe in blotting one of the incorporated nucleotides provided in the reaction is radiolabeled, or a fluorophore can be attached instead for fluorescent labelling, or is used as an antigen for immunodetection.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-50 DNA Grade can be used in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides. Sephadex G-50 can be packed into empty MicroSpin columns for use in these applications.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length. Alternatively, Sephadex G-25 can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nBoth types of Sephadex are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or in radioactive labeling.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 DNA Grade and Sephadex G-50 DNA Grade are sold separately.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775945593123,"sku":"CYTI17085502","price":668.7,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/17085502.png?v=1781219539"},{"product_id":"amersham-nap-10-columns","title":"Amersham NAP-10 Columns","description":"\u003cp\u003eNAP DNA columns are disposable DNA purification size exclusion columns, prepacked with Sephadex G-25 DNA Grade which require only gravity to run.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid and efficient purification of DNA and oligonucleotides (= 10-mers) in less than 15 minutes by gravity flow\u003cbr\u003e\r\n•Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL (NAP-25)\u003cbr\u003e\r\n•Useful for small-scale purification, oligo purification, desalting, and buffer exchange\u003cbr\u003e\r\n\u003cbr\u003e\r\nNAP DNA purification columns with Sephadex G-25 DNA Grade can be used for any DNA greater than 10 bases in length. These disposable DNA purification columns are ideal for the purification of oligonucleotides or very small DNA fragments following synthesis or a DNA labeling reaction, and for downstream applications such as PCR amplification and sequencing.\u003cbr\u003e\r\n\u003cbr\u003e\r\nEach package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration, washing, and sample elution.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775947329827,"sku":"CYTI17085401","price":309.1,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/17085201.jpg?v=1789940426"},{"product_id":"autoseq-g-50-50-columns","title":"AutoSeq G-50, 50 columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nAutoSeq G-50 columns are specifically designed to remove fluorescent dye-terminators from cycle sequencing reactions prior to analysis on automated sequencers.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated DNA sequencing\u003cbr\u003e\r\n•Columns are prepacked with Sephadex G-50 DNA Grade F\u003cbr\u003e\r\n•Columns are pre-equilibrated in double-distilled water with 0.05% Kathon CG\/ICP Biocide added as a preservative\u003cbr\u003e\r\n•Ready-to-use columns enable sample application through to collection of purified product in less than 4 min\u003cbr\u003e\r\n•4 minutes\u003cbr\u003e\r\n\u003cbr\u003e\r\nEffective DNA purification is essential for high-quality results on automated DNA sequencing since residual dye-terminators can obscure data.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50 DNA Grade F, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artefacts on salt-sensitive automated DNA sequencers.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775947755811,"sku":"CYTI27534001","price":277.5,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27534003_61cd0ee3-c635-46ac-a59e-29443b1abd23.jpg?v=1789940742"},{"product_id":"amersham-nap-10-columns-1","title":"Amersham NAP-10 Columns","description":"\u003cp\u003eNAP DNA columns are disposable DNA purification size exclusion columns, prepacked with Sephadex G-25 DNA Grade which require only gravity to run.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid and efficient purification of DNA and oligonucleotides (= 10-mers) in less than 15 minutes by gravity flow\u003cbr\u003e\r\n•Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL (NAP-25)\u003cbr\u003e\r\n•Useful for small-scale purification, oligo purification, desalting, and buffer exchange\u003cbr\u003e\r\n\u003cbr\u003e\r\nNAP DNA purification columns with Sephadex G-25 DNA Grade can be used for any DNA greater than 10 bases in length. These disposable DNA purification columns are ideal for the purification of oligonucleotides or very small DNA fragments following synthesis or a DNA labeling reaction, and for downstream applications such as PCR amplification and sequencing.\u003cbr\u003e\r\n\u003cbr\u003e\r\nEach package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration, washing, and sample elution.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775948312867,"sku":"CYTI17085402","price":606.4,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/17085201_4d716f81-a5b5-4405-ab41-7fc97c4f9392.jpg?v=1789940432"},{"product_id":"amersham-microspin-g-25-columns","title":"Amersham MicroSpin G-25 Columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nMicroSpin G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Excellent for rapid buffer exchange\/desalting: for example, of PCR amplification products and other DNAs in a volume of 10 to 100 µL using spin-column chromatography\u003cbr\u003e\r\n•Excellent for rapid DNA purification: especially newly synthesized oligonucleotides \u0026gt; 10-mers in 100 to 150 µL of deprotection solution\u003cbr\u003e\r\n•Convenient: Prepacked with Sephadex G-25 DNA Grade and pre-equilibrated in distilled wa-ter containing 0.05% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Ready to use: requires less than 4 minutes from sample application to collection of purified oligonucleotides\u003cbr\u003e\r\n•Tested in assays: including nickase, single- and double-stranded exonuclease, and RNase\u003cbr\u003e\r\n•Use additionally: for desalting\/buffer exchange of DNA and removal of unincorporated radi-onucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin G-25 columns for rapid DNA purification in desalting and buffer exchange applications\u003cbr\u003e\r\nMicroSpin G-25 columns are appropriate for use with DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or labeling reactions. They will not remove or denature enzymes.\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin G-25 columns contain Sephadex G-25 DNA Grade F resin. They allow DNA purification via gel filtration. Gel filtration resins do not exhibit a fixed exclusion limit when used in a spin-column format. In spin-column chromatography, the observed exclusion properties that allow the product to pass through the gel while the smaller impurities are retained depends on experimental factors. These factors include the resin used, sample volume, product size, and the g-forces used.\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe resin used in these columns, Sephadex G-25 has an exclusion limit of approximately Mr 5000. With a spin protocol this resin can be used for any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-100 for larger molecules. Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules \u0026gt;30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length. Sephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAll these Sephadex types are therefore highly suitable for the purification of oligonucleotides or small DNA fragments following synthesis or labeling reactions.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25, G-50 DNA Grade and Sephadex G-100 are all sold separately.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775948673315,"sku":"CYTI27532501","price":368.7,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27532501.jpg?v=1789940751"},{"product_id":"gfx-96-pcr-purification-kit-10-x-96-well-plates","title":"GFX 96 PCR Purification Kit","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\nKit uses glass fiber matrix technology in a 96-well format for highly efficient purification of PCR products. DNA fragments from PCR are captured by the matrix in presence of a chaotropic salt and contaminants are removed by washing matrix.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For the purification of up to 96 PCR products (0.1 to 10 kb) simultaneously in as little as 15 min.\u003cbr\u003e\r\n•High yields of pure DNA recovered in a small volume of water or a low ionic strength buffer.\u003cbr\u003e\r\n•Typical recoveries are \u0026gt; 85% for PCR products 100 bp to 10 kb in length; salt removal typically = 99%.\u003cbr\u003e\r\n•Prepacked glass fiber matrix 96-well plates are validated for use with Macherey-Nagel NucleoVac 96 vacuum manifold.\u003cbr\u003e\r\n•Avoids ethanol precipitations and hazardous organic extractions.\u003cbr\u003e\r\n•Purified DNA is ready for use in most applications, including fluorescent sequencing, microarrays, labeling, hybridization, ligation, and transformation.\u003cbr\u003e\r\n\u003cbr\u003e\r\nGFX 96 Purification Kit utilizes glass fiber matrix technology in a 96-well format for highly efficient purification of PCR products. Fragments from PCR are captured by the matrix in the presence of a chaotropic salt, and contaminants are conveniently removed by washing the matrix with a buffered ethanol solution.\u003cbr\u003e\r\n\u003cbr\u003e\r\nGFX 96 PCR Purification Kit contains the following components in sufficient quantities: binding plates, wash plates, collection plates, color-coded bottles of capture buffer, wash buffer, and a choice of two elution buffers (Tris-HCl and sterile water), and an instruction booklet.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775949492515,"sku":"CYTI28903445","price":2413.6,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/28903445.jpg?v=1789944616"},{"product_id":"probequant-g-50-micro-columns","title":"ProbeQuant G-50 Micro Columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for the preparation of DNA probes, separating them from unincorporated labeled nucleotides.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid purification of labeled DNA (= 20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 µL using spin-column chromatography\u003cbr\u003e\r\n•Prepacked with Sephadex G-50 DNA Grade and pre-equilibrated in STE containing 0.15% Kathon CG\/ICP Biocide for convenience\u003cbr\u003e\r\n•Ready to use and requires less than 4 minutes from sample application to collection of purified product\u003cbr\u003e\r\n•Designed for use in a microcentrifuge\u003cbr\u003e\r\n•Tested in assays: nickase, single and double-stranded exonuclease and RNase\u003cbr\u003e\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\nProbeQuant G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labeled probes from unincorporated labeled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation.\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe columns are designed for use in a microcentrifuge, come prepacked with Sephadex G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 µL to 50 µL.\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe resin used in these columns, Sephadex G-50 DNA Grade, can be used in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin columns packed with Sephadex G-50 DNA Grade are ideal for the rapid purification of DNA for use in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides .\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established size exclusion chromatography resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAlternatively, Sephadex G-25 or Sephadex G-100 can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAll these Sephadex types are therefore suitable for the purification of oligonucleotides or small DNA fragments following synthesis or a labeling reaction.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775949885731,"sku":"CYTI28903408","price":368.7,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/28903408.jpg?v=1789944619"},{"product_id":"microspin-columns-100-pieces","title":"MicroSpin Columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nDesigned for the rapid purification of DNA, in conjunction with Sephadex G-25 DNA Grade or Sephadex G-50 DNA Grade, for desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.\u003cbr\u003e\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid buffer exchange\/desalting of PCR products and other DNA samples in a volume of 10 to 100 µL using spin-column chromatography and Sephadex G-25 DNA Grade (not included)\u003cbr\u003e\r\n•Excellent for rapid purification of newly synthesized oligonucleotides \u0026gt; 10-mers in 100 to 150 µL of deprotection solution using spin-column chromatography\u003cbr\u003e\r\n•Flexible for your experiments, allowing differing amounts of Sephadex\u003cbr\u003e\r\n•Can also be used for desalting\/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nBoth types of Sephadex are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775950311715,"sku":"CYTI27356501","price":295.5,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27356501.jpg?v=1789940758"},{"product_id":"amersham-nick-columns-20","title":"Amersham NICK Columns -20","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\n\u003cbr\u003e\r\nNICK Columns are used for the purification of nick-translated DNA fragments and for separation of any labeled probe from unincorporated labeled nucleotides.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid purification of labeled DNA (= 20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography.\u003cbr\u003e\r\n•Prepacked with Sephadex G-50 DNA Grade (see Sephadex G-50 DNA Grade and Sephadex G-100 DNA Grade) in distilled water with 0.15% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Can accommodate sample volumes up to 100 µl\u003cbr\u003e\r\n\u003cbr\u003e\r\nNICK columns are ready for immediate use and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA molecules and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered and can then be used in DNA hybridization protocols. Maximum sample volume is 100 µL.\u003cbr\u003e\r\n\u003cbr\u003e\r\nNick translation is a technique in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques. It can also be used for radioactive labeling in techniques such as Southern blotting.\u003cbr\u003e\r\n\u003cbr\u003e\r\nThis process is called nick translation because the DNA to be labelled is treated with DNase to produce single-stranded \"nicks.\" This is followed by replacement in nicked sites by DNA polymerase I, which elongates the 3' end, removing nucleotides by 5'-3' exonuclease activity, and replacing them with dNTPs. To label a DNA fragment for use as a probe in blotting one of the incorporated nucleotides provided in the reaction is radiolabeled, or a fluorophore can be attached instead for fluorescent labelling, or is used as an antigen for immunodetection.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-50 DNA Grade can be used in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides. Sephadex G-50 can be packed into empty MicroSpin columns for use in these applications.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length. Alternatively, Sephadex G-25 can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nBoth types of Sephadex are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or in radioactive labeling.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 DNA Grade and Sephadex G-50 DNA Grade are sold separately.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775950410019,"sku":"CYTI17085501","price":310.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/17085501.png?v=1781219542"},{"product_id":"exoprostar-20-reactions","title":"ExoProStar, 20 reactions (US78220)","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides.\u003cbr\u003e\r\n•Enzymes provided in two separate tubes; just two simple pipetting steps are needed to prepare the reaction.\u003cbr\u003e\r\n•Fast 30 min protocol.\u003cbr\u003e\r\n•Scalable for different reaction sizes.\u003cbr\u003e\r\n•No loss of PCR product.\u003cbr\u003e\r\n•Easy to automate.\u003cbr\u003e\r\n•Complete heat inactivation of the enzymes within 15 min.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. ExoProStar improves digestion efficiency with no degradation of the target PCR product. Your sample is completely intact and ready for immediate use in manual or automated processes.\u003cbr\u003e\r\n\u003cbr\u003e\r\nFor the convenience of a single, stable enzyme mix with just one pipetting step we also offer the ExoProStar 1-Step (not sold in the USA, Canada or Japan).\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775951294755,"sku":"CYTI29002088","price":64.9,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/29002090_6e09b6b2-6625-4125-b77f-4219df2a0f0c.jpg?v=1789945225"},{"product_id":"gfx-pcr-dna-and-gel-band-purification-kit-100-purifications","title":"GFX PCR DNA and Gel Band Purification Kit, 100 purifications","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\nDesigned for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 µl or agarose gel slices up to 900 mg.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.\u003cbr\u003e\r\n•Fast and easy-to-use method with less than 10 min hands-on time.\u003cbr\u003e\r\n•Flexible 10 to 50 µl elution volume for different DNA concentration needs.\u003cbr\u003e\r\n•Improved column design with increased maximum volume, holding of up to 800 µl liquid volume (950 µl without a cap).\u003cbr\u003e\r\n•Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.\u003cbr\u003e\r\n•Addition of a visual color indicator to the capture buffer ensures optimal pH for maximum DNA binding and recovery.\u003cbr\u003e\r\n•Two choices of elution buffer provided to fit downstream applications.\u003cbr\u003e\r\n•Color-coded for ease of use.\u003cbr\u003e\r\n\u003cbr\u003e\r\n\u003cbr\u003e\r\nGFX PCR DNA and Gel Band Purification Kit is designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 µl or agarose gel slices up to 900 mg. The kit combines a versatile chaotropic buffer with a glass fiber matrix supported in a spin column for the purification of DNA from both solution and agarose gel. Typical recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution. DNA purity is exceptional; 99.5% of contaminants are removed.\u003cbr\u003e\r\n\u003cbr\u003e\r\nGFX PCR DNA and Gel Band Purification Kit contains the following components in sufficient quantities: GFX columns, collection tubes, color-coded bottles of capture buffer, wash buffer, and two elution buffers (Tris-HCl and sterile water), and an instruction booklet.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775951393059,"sku":"CYTI28903470","price":300.9,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/28903470.jpg?v=1789944621"},{"product_id":"amersham-microspin-g-50-columns","title":"Amersham MicroSpin G-50 Columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nMicroSpin G-50 columns are designed for the rapid purification of DNA in desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid purification of labeled DNA (DNA probes of \u0026gt; 20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 µL using spin-column chromatography.\u003cbr\u003e\r\n•Columns are prepacked with Sephadex G-50 DNA Grade pre-equilibrated in TE buffer with 0.05% Kathon CG\/ICP Biocide.\u003cbr\u003e\r\n•Product recovery will be at least 10% lower than that from ProbeQuant G-50 Micro Columns.\u003cbr\u003e\r\n•Can also be used for buffer exchange\/desalting of enzymatic reactions (10 to 100 µl) following heat denaturation and phenol extraction.\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin G-50 Columns, rapid purification of DNA requiring clean-up\u003cbr\u003e\r\nGood product yield and purity is obtained with sample volumes from 12-50 µL. They are suitable for any DNA greater than 20 bases in length and will not remove or denature enzymes.\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe resin used in these columns, Sephadex G-50 DNA Grade, can be used for a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established size exclusion resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAlternatively, Sephadex G-25 or Sephadex G-100 can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAll these Sephadex types are therefore suitable for the purification of oligonucleotides or small DNA fragments following synthesis or a labeling reaction.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 DNA Grade, Sephadex G-50 DNA Grade, and Sephadex G-100 are all sold separately.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775951950115,"sku":"CYTI27533001","price":368.7,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27533001.jpg?v=1789940767"},{"product_id":"autoseq-g-50-1000-columns","title":"AutoSeq G-50, 1000 columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nAutoSeq G-50 columns are specifically designed to remove fluorescent dye-terminators from cycle sequencing reactions prior to analysis on automated sequencers.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated DNA sequencing\u003cbr\u003e\r\n•Columns are prepacked with Sephadex G-50 DNA Grade F\u003cbr\u003e\r\n•Columns are pre-equilibrated in double-distilled water with 0.05% Kathon CG\/ICP Biocide added as a preservative\u003cbr\u003e\r\n•Ready-to-use columns enable sample application through to collection of purified product in less than 4 min\u003cbr\u003e\r\n•4 minutes\u003cbr\u003e\r\n\u003cbr\u003e\r\nEffective DNA purification is essential for high-quality results on automated DNA sequencing since residual dye-terminators can obscure data.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50 DNA Grade F, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artefacts on salt-sensitive automated DNA sequencers.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775952015651,"sku":"CYTI27534003","price":3122.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27534003_f86da99d-5f9e-4e0e-ac6a-d95431597775.jpg?v=1789940769"},{"product_id":"exoprostar-100-reactions","title":"ExoProStar, 100 reactions (US78210)","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides.\u003cbr\u003e\r\n•Enzymes provided in two separate tubes; just two simple pipetting steps are needed to prepare the reaction.\u003cbr\u003e\r\n•Fast 30 min protocol.\u003cbr\u003e\r\n•Scalable for different reaction sizes.\u003cbr\u003e\r\n•No loss of PCR product.\u003cbr\u003e\r\n•Easy to automate.\u003cbr\u003e\r\n•Complete heat inactivation of the enzymes within 15 min.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. ExoProStar improves digestion efficiency with no degradation of the target PCR product. Your sample is completely intact and ready for immediate use in manual or automated processes.\u003cbr\u003e\r\n\u003cbr\u003e\r\nFor the convenience of a single, stable enzyme mix with just one pipetting step we also offer the ExoProStar 1-Step (not sold in the USA, Canada or Japan).\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775952376099,"sku":"CYTI29002090","price":114.4,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/29002090.jpg?v=1789945524"},{"product_id":"exoprostar-2000-reactions","title":"ExoProStar, 2000 reactions (US78212)","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides.\u003cbr\u003e\r\n•Enzymes provided in two separate tubes; just two simple pipetting steps are needed to prepare the reaction.\u003cbr\u003e\r\n•Fast 30 min protocol.\u003cbr\u003e\r\n•Scalable for different reaction sizes.\u003cbr\u003e\r\n•No loss of PCR product.\u003cbr\u003e\r\n•Easy to automate.\u003cbr\u003e\r\n•Complete heat inactivation of the enzymes within 15 min.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. ExoProStar improves digestion efficiency with no degradation of the target PCR product. Your sample is completely intact and ready for immediate use in manual or automated processes.\u003cbr\u003e\r\n\u003cbr\u003e\r\nFor the convenience of a single, stable enzyme mix with just one pipetting step we also offer the ExoProStar 1-Step (not sold in the USA, Canada or Japan).\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775952572707,"sku":"CYTI29002092","price":1616.6,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/29002090_349a28d1-9c5d-4865-b93e-7649c9d3692f.jpg?v=1789945528"},{"product_id":"exoprostar-500-reactions","title":"ExoProStar, 500 reactions (US78211)","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides.\u003cbr\u003e\r\n•Enzymes provided in two separate tubes; just two simple pipetting steps are needed to prepare the reaction.\u003cbr\u003e\r\n•Fast 30 min protocol.\u003cbr\u003e\r\n•Scalable for different reaction sizes.\u003cbr\u003e\r\n•No loss of PCR product.\u003cbr\u003e\r\n•Easy to automate.\u003cbr\u003e\r\n•Complete heat inactivation of the enzymes within 15 min.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. ExoProStar improves digestion efficiency with no degradation of the target PCR product. Your sample is completely intact and ready for immediate use in manual or automated processes.\u003cbr\u003e\r\n\u003cbr\u003e\r\nFor the convenience of a single, stable enzyme mix with just one pipetting step we also offer the ExoProStar 1-Step (not sold in the USA, Canada or Japan).\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775952703779,"sku":"CYTI29002091","price":455.5,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/29002090_1a5d0a0d-9eb3-4779-9e7f-99f51edfc7cc.jpg?v=1789945531"},{"product_id":"exoprostar-1-step-5000-reactions","title":"ExoProStar 1-Step, 5000 reactions","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. For maximum convenience comes in a single tube.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•One tube with one mix of enzymes delivering PCR and sequence reaction clean-up\u003cbr\u003e\r\n•Just one pipetting step to set up your reaction\u003cbr\u003e\r\n•Fast 30-minute, hands-free protocol\u003cbr\u003e\r\n•100% recovery of PCR product\u003cbr\u003e\r\n•Scalable for different reaction sizes\u003cbr\u003e\r\n•Automation friendly\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar™ is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar™ 1-Step PCR and Sequence Reaction Clean-Up reagent provides a simple, single-step method for removing unincorporated primers and nucleotides prior to downstream analysis. Exonuclease I and Alkaline Phosphatase enzymes are combined in a single, stable mix requiring just one pipetting step to set up the clean-up reaction.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar™ 1-Step is based on Cytiva''s founding patents for the clean-up of PCR reactions using Exonuclease 1 and Alkaline Phosphatase. This 1-step version is not available in Canada and Japan. Instead, we offer a version with the enzymes in two separate tubes, the ExoProStar™, and the faster version, ExoProStar™ S.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775952933155,"sku":"CYTI29010367","price":5618.7,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/Exoprostar.jpg?v=1789945533"},{"product_id":"amersham-nap-5-columns","title":"Amersham NAP-5 Columns","description":"\u003cp\u003eNAP DNA columns are disposable DNA purification size exclusion columns, prepacked with Sephadex G-25 DNA Grade which require only gravity to run.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid and efficient purification of DNA and oligonucleotides (= 10-mers) in less than 15 minutes by gravity flow\u003cbr\u003e\r\n•Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL (NAP-25)\u003cbr\u003e\r\n•Useful for small-scale purification, oligo purification, desalting, and buffer exchange\u003cbr\u003e\r\n\u003cbr\u003e\r\nNAP DNA purification columns with Sephadex G-25 DNA Grade can be used for any DNA greater than 10 bases in length. These disposable DNA purification columns are ideal for the purification of oligonucleotides or very small DNA fragments following synthesis or a DNA labeling reaction, and for downstream applications such as PCR amplification and sequencing.\u003cbr\u003e\r\n\u003cbr\u003e\r\nEach package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration, washing, and sample elution.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775953326371,"sku":"CYTI17085301","price":281.1,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/17085201_8d2ec281-deb9-439a-b152-4f3cf595db93.jpg?v=1789940124"},{"product_id":"exoprostar-1-step-100-reactions","title":"ExoProStar 1-Step, 100 reactions","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. For maximum convenience comes in a single tube.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•One tube with one mix of enzymes delivering PCR and sequence reaction clean-up\u003cbr\u003e\r\n•Just one pipetting step to set up your reaction\u003cbr\u003e\r\n•Fast 30-minute, hands-free protocol\u003cbr\u003e\r\n•100% recovery of PCR product\u003cbr\u003e\r\n•Scalable for different reaction sizes\u003cbr\u003e\r\n•Automation friendly\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar™ is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar™ 1-Step PCR and Sequence Reaction Clean-Up reagent provides a simple, single-step method for removing unincorporated primers and nucleotides prior to downstream analysis. Exonuclease I and Alkaline Phosphatase enzymes are combined in a single, stable mix requiring just one pipetting step to set up the clean-up reaction.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar™ 1-Step is based on Cytiva''s founding patents for the clean-up of PCR reactions using Exonuclease 1 and Alkaline Phosphatase. This 1-step version is not available in Canada and Japan. Instead, we offer a version with the enzymes in two separate tubes, the ExoProStar™, and the faster version, ExoProStar™ S.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775953654051,"sku":"CYTI29010361","price":160.4,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/Exoprostar_09a6a8a3-fbb0-425b-acca-2d3d77973a98.jpg?v=1789945834"},{"product_id":"amersham-nap-25-columns","title":"Amersham NAP-25 Columns","description":"\u003cp\u003eNAP DNA columns are disposable DNA purification size exclusion columns, prepacked with Sephadex G-25 DNA Grade which require only gravity to run.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid and efficient purification of DNA and oligonucleotides (= 10-mers) in less than 15 minutes by gravity flow\u003cbr\u003e\r\n•Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL (NAP-25)\u003cbr\u003e\r\n•Useful for small-scale purification, oligo purification, desalting, and buffer exchange\u003cbr\u003e\r\n\u003cbr\u003e\r\nNAP DNA purification columns with Sephadex G-25 DNA Grade can be used for any DNA greater than 10 bases in length. These disposable DNA purification columns are ideal for the purification of oligonucleotides or very small DNA fragments following synthesis or a DNA labeling reaction, and for downstream applications such as PCR amplification and sequencing.\u003cbr\u003e\r\n\u003cbr\u003e\r\nEach package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration, washing, and sample elution.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775953883427,"sku":"CYTI17085202","price":799.7,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/17085201_153c4058-6f75-417e-bbdb-3c0827a539f0.jpg?v=1789940129"},{"product_id":"exoprostar-1-step-500-reactions","title":"ExoProStar 1-Step, 500 reactions","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. For maximum convenience comes in a single tube.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•One tube with one mix of enzymes delivering PCR and sequence reaction clean-up\u003cbr\u003e\r\n•Just one pipetting step to set up your reaction\u003cbr\u003e\r\n•Fast 30-minute, hands-free protocol\u003cbr\u003e\r\n•100% recovery of PCR product\u003cbr\u003e\r\n•Scalable for different reaction sizes\u003cbr\u003e\r\n•Automation friendly\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar™ is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar™ 1-Step PCR and Sequence Reaction Clean-Up reagent provides a simple, single-step method for removing unincorporated primers and nucleotides prior to downstream analysis. Exonuclease I and Alkaline Phosphatase enzymes are combined in a single, stable mix requiring just one pipetting step to set up the clean-up reaction.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar™ 1-Step is based on Cytiva''s founding patents for the clean-up of PCR reactions using Exonuclease 1 and Alkaline Phosphatase. This 1-step version is not available in Canada and Japan. Instead, we offer a version with the enzymes in two separate tubes, the ExoProStar™, and the faster version, ExoProStar™ S.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775954342179,"sku":"CYTI29010363","price":691.3,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/Exoprostar_abec26eb-5e4d-4880-bda1-f44bbf4f3833.jpg?v=1789945542"},{"product_id":"exoprostar-1-step-2000-reactions","title":"ExoProStar 1-Step, 2000 reactions","description":"\u003cp\u003eOverview:\u003cbr\u003e\r\nEnzymatic PCR clean up technology with Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. For maximum convenience comes in a single tube.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•One tube with one mix of enzymes delivering PCR and sequence reaction clean-up\u003cbr\u003e\r\n•Just one pipetting step to set up your reaction\u003cbr\u003e\r\n•Fast 30-minute, hands-free protocol\u003cbr\u003e\r\n•100% recovery of PCR product\u003cbr\u003e\r\n•Scalable for different reaction sizes\u003cbr\u003e\r\n•Automation friendly\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar™ is our new name for this PCR clean-up technology. No change in the formulation, so you get the same great product, and same great benefits!\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe ExoProStar™ 1-Step PCR and Sequence Reaction Clean-Up reagent provides a simple, single-step method for removing unincorporated primers and nucleotides prior to downstream analysis. Exonuclease I and Alkaline Phosphatase enzymes are combined in a single, stable mix requiring just one pipetting step to set up the clean-up reaction.\u003cbr\u003e\r\n\u003cbr\u003e\r\nExoProStar™ 1-Step is based on Cytiva''s founding patents for the clean-up of PCR reactions using Exonuclease 1 and Alkaline Phosphatase. This 1-step version is not available in Canada and Japan. Instead, we offer a version with the enzymes in two separate tubes, the ExoProStar™, and the faster version, ExoProStar™ S.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775954997539,"sku":"CYTI29176221","price":2449.7,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/Exoprostar_d9722549-55e9-4b01-958b-be7f1a4e912a.jpg?v=1789945842"},{"product_id":"amersham-nap-25-columns-1","title":"Amersham NAP-25 Columns","description":"\u003cp\u003eNAP DNA columns are disposable DNA purification size exclusion columns, prepacked with Sephadex G-25 DNA Grade which require only gravity to run.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid and efficient purification of DNA and oligonucleotides (= 10-mers) in less than 15 minutes by gravity flow\u003cbr\u003e\r\n•Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL (NAP-25)\u003cbr\u003e\r\n•Useful for small-scale purification, oligo purification, desalting, and buffer exchange\u003cbr\u003e\r\n\u003cbr\u003e\r\nNAP DNA purification columns with Sephadex G-25 DNA Grade can be used for any DNA greater than 10 bases in length. These disposable DNA purification columns are ideal for the purification of oligonucleotides or very small DNA fragments following synthesis or a DNA labeling reaction, and for downstream applications such as PCR amplification and sequencing.\u003cbr\u003e\r\n\u003cbr\u003e\r\nEach package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration, washing, and sample elution.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775955357987,"sku":"CYTI17085201","price":375.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/17085201_c89b7e23-4785-4b84-84df-ec22d22586be.jpg?v=1789940135"},{"product_id":"amersham-microspin-g-50-columns-1","title":"Amersham MicroSpin G-50 Columns","description":"\u003cp\u003eOverview\u003cbr\u003e\r\nMicroSpin G-50 columns are designed for the rapid purification of DNA in desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid purification of labeled DNA (DNA probes of \u0026gt; 20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 µL using spin-column chromatography.\u003cbr\u003e\r\n•Columns are prepacked with Sephadex G-50 DNA Grade pre-equilibrated in TE buffer with 0.05% Kathon CG\/ICP Biocide.\u003cbr\u003e\r\n•Product recovery will be at least 10% lower than that from ProbeQuant G-50 Micro Columns.\u003cbr\u003e\r\n•Can also be used for buffer exchange\/desalting of enzymatic reactions (10 to 100 µl) following heat denaturation and phenol extraction.\u003cbr\u003e\r\n\u003cbr\u003e\r\nMicroSpin G-50 Columns, rapid purification of DNA requiring clean-up\u003cbr\u003e\r\nGood product yield and purity is obtained with sample volumes from 12-50 µL. They are suitable for any DNA greater than 20 bases in length and will not remove or denature enzymes.\u003cbr\u003e\r\n\u003cbr\u003e\r\nThe resin used in these columns, Sephadex G-50 DNA Grade, can be used for a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established size exclusion resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAlternatively, Sephadex G-25 or Sephadex G-100 can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA.\u003cbr\u003e\r\n\u003cbr\u003e\r\nAll these Sephadex types are therefore suitable for the purification of oligonucleotides or small DNA fragments following synthesis or a labeling reaction.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 DNA Grade, Sephadex G-50 DNA Grade, and Sephadex G-100 are all sold separately.\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775955947811,"sku":"CYTI27533002","price":1468.4,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/27533001_efcfbde7-faaf-4663-b4b4-ed64170df0f6.jpg?v=1789940780"},{"product_id":"amersham-nap-5-columns-1","title":"Amersham NAP-5 Columns","description":"\u003cp\u003eNAP DNA columns are disposable DNA purification size exclusion columns, prepacked with Sephadex G-25 DNA Grade which require only gravity to run.\u003cbr\u003e\r\n\u003cbr\u003e\r\n•For rapid and efficient purification of DNA and oligonucleotides (= 10-mers) in less than 15 minutes by gravity flow\u003cbr\u003e\r\n•Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG\/ICP Biocide\u003cbr\u003e\r\n•Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL (NAP-25)\u003cbr\u003e\r\n•Useful for small-scale purification, oligo purification, desalting, and buffer exchange\u003cbr\u003e\r\n\u003cbr\u003e\r\nNAP DNA purification columns with Sephadex G-25 DNA Grade can be used for any DNA greater than 10 bases in length. These disposable DNA purification columns are ideal for the purification of oligonucleotides or very small DNA fragments following synthesis or a DNA labeling reaction, and for downstream applications such as PCR amplification and sequencing.\u003cbr\u003e\r\n\u003cbr\u003e\r\nEach package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration, washing, and sample elution.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.\u003cbr\u003e\r\n\u003cbr\u003e\r\n**Image is a Sample Only**\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"Default Title","offer_id":54775956209955,"sku":"CYTI17085302","price":606.4,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/17085201_87e406a7-6510-4903-b4f5-a443625294c8.jpg?v=1789940137"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/collections\/CYTIVA_20logo_96902564-4a22-4e6f-b69f-d06ca618e6b8.jpg?v=1772146027","url":"https:\/\/onelab.co.nz\/collections\/pcr-clean-up-and-size-selection.oembed?page=2","provider":"Onelab","version":"1.0","type":"link"}