{"title":"Enzymes","description":"","products":[{"product_id":"pectinase-en","title":"Pectinase (en)","description":"\u003ch3\u003eIntended use:\u003c\/h3\u003e\r\nAn enzyme for the clarification of cloudy apple juice and apple purée prior to patulin analysis.\r\n\u003cbr\u003e\r\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\r\n\u003cul\u003e \u003cbr\u003e\r\n\u003cli\u003eTest format: 100 determinations\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eSample preparation: Sample is extracted with ethyl acetate and then cleaned-up by extraction with sodium carbonate solution. Samples are pre-treated with pectinase enzyme. The ethyl acetate extract is dried with anhydrous sodium sulphate. After evaporation of the solvent, the toxin is quantitatively determined by LC with UV detection. The total sample extraction and clean-up takes approximately 2 hours to perform\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eIncubation time: 2 hours at 40 °C, then centrifugation for 5 minutes or leave to settle overnight at room temperature, then centrifugation for 5 minutes.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eValidated matrices: Cloudy apple juice and apple purée.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eEvaluation: HPLC or LC-MS\/MS.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eApprovals: Pectinase is used as part of AOAC method 2000.02 for analysis of patulin in cloudy apple juice and purée.\u003c\/li\u003e\r\n\u003cbr\u003e\r\n\u003cli\u003eRelated products: EASIMIP™ PATULIN \u003c\/li\u003e\r\n\u003c\/ul\u003e\u003cbr\u003e\r\n\r\n\u003ch3\u003eGeneral Information:\u003c\/h3\u003e\r\n\u003cbr\u003e\r\nFollowing AOAC method 2000.02 for patulin in apple juice (MacDonald et al.: Journal of AOAC International. Vol 83: No 6, 2000.) the apple juice is treated with pectinase enzyme to clarify the sample prior to extraction. Clear apple juice does not require the addition of pectinase prior to extraction.\r\n\u003cbr\u003e\u003ch3\u003eBenefits:\u003c\/h3\u003e\r\n\u003cul\u003e\r\n \t\u003cli\u003ePectinase is used as part of AOAC method 2000.02 for analysis of patulin in cloudy apple juice and purée.\u003c\/li\u003e\r\n \t\u003cli\u003eEnables the analysis of cloudy apple juice and purée. Available as a ready-to-use solution. \u003c\/li\u003e\r\n \t\u003cli\u003eEach batch of pectinase is accompanied by a certificate of conformity.\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"R-Biopharm","offers":[{"title":"Default Title","offer_id":53657911394595,"sku":"RBIORBRP129","price":318.9,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/RBRP129.jpg?v=1779864261"},{"product_id":"e-amgdf-100ml-amyloglucosidase-a-niger-100ml","title":"E-AMGDF-100ML Amyloglucosidase (A.niger) 100ml","description":"\u003cp\u003eHigh purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.\u003cbr\u003e\r\n\u003cbr\u003e\r\nFor use in Megazyme Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.\u003cbr\u003e\r\n\u003cbr\u003e\r\nE-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54217141715235,"sku":"MEGAE-AMGDF-100ML","price":876.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-AMGDF.jpg?v=1790299214"},{"product_id":"e-frmxpd-fructanase-mixture-20-000u","title":"E-FRMXPD Fructanase Mixture - 20,000U","description":"\u003cp\u003ePowder (freeze dried)\u003cbr\u003e\r\n\u003cbr\u003e\r\nFructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.\u003cbr\u003e\r\n\u003cbr\u003e\r\nComponents: exo-inulinase 20,000 U (on kestose, at 40°C), endo-inulinase 1000 U (on fructan at 40°C), a-galactosidase 0.70 U (on p-nitrophenyl a-galactoside at 40°C), ß-Glucanase 1.25 U (on ß-glucan at 40°C) and pectinase 5.5 U (on pectin at 40°C).\u003cbr\u003e\r\n\u003cbr\u003e\r\nNote: This product has been purified to remove a-galactosidase, ß-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).\u003cbr\u003e\r\n\u003cbr\u003e\r\nFructanase and sucrase (E-SUCR) are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54217142108451,"sku":"MEGAE-FRMXPD","price":622.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-FRMXPD.jpg?v=1790291716"},{"product_id":"amyloglucosidase-aspergillus-niger","title":"E-AMGDF-40ML Amyloglucosidase (Aspergillus niger)","description":"\u003cp\u003eHigh purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.\u003cbr\u003e\r\n\u003cbr\u003e\r\nFor use in Megazyme Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.\u003cbr\u003e\r\n\u003cbr\u003e\r\nE-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54217142403363,"sku":"MEGAE-AMGDF-40ML","price":438.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-AMGDF_eb45c811-2327-4a77-91d4-55ca9ac9357f.jpg?v=1788445850"},{"product_id":"e-bglan-b-galactosidase-a-niger-8ku","title":"E-BGLAN B-Galactosidase (A.niger) 8KU","description":"\u003cp\u003eHigh purity ß-Galactosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54217142665507,"sku":"MEGAE-BGLAN","price":478.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-BGLAN.jpg?v=1788445855"},{"product_id":"a-amylase-kit","title":"E-ANAAM a-Amylase Kit","description":"\u003cp\u003eHigh purity a-Amylase (Aspergillus oryzae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54217142829347,"sku":"MEGAE-ANAAM","price":450.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-ANAAM.jpg?v=1790294120"},{"product_id":"e-amgpu-amyloglucosidase-rhizopus-sp-5ku","title":"E-AMGPU Amyloglucosidase (Rhizopus sp.) 5KU","description":"\u003cp\u003eHigh purity Amyloglucosidase (Rhizopus sp.) for use in research, biochemical enzyme assays and analytical testing applications.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54333591093539,"sku":"MEGAE-AMGPU","price":561.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-AMGPU.jpg?v=1781229711"},{"product_id":"e-bstaa-a-amylase-thermostable-bacillus-sp-10mls","title":"E-BSTAA a-Amylase (Thermostable) (Bacillus sp.) 10mls","description":"\u003cp\u003eHigh purity a-Amylase (Thermostable) (Bacillus sp.) for use in research, biochemical enzyme assays and analytical testing applications. This enzyme is considerably more thermostable than related enzymes such as the heat stable a-amylase (Bacillus licheniformis, E-BLAAM).\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54333591421219,"sku":"MEGAE-BSTAA","price":315.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-BSTAA.jpg?v=1789482634"},{"product_id":"e-frmxlq-fructanase-mixture-purified-10ml","title":"E-FRMXLQ Fructanase Mixture (Purified) 10ml","description":"\u003cp\u003eHigh purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and analytical testing applications.\u003cbr\u003e\r\n\u003cbr\u003e\r\nFor Fructan Determination (Liquid).\u003cbr\u003e\r\n\u003cbr\u003e\r\nFructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.\u003cbr\u003e\r\n\u003cbr\u003e\r\nComponents: exo-inulinase 2,000 U\/ml (on kestose, at 40oC), endo-inulinase ˜ 100 U\/ml (on fructan at 40oC), a-galactosidase \u0026lt; 0.08 U\/ml (on polygalacturonic acid at 40oC), ß-Glucanase \u0026lt; 0.04 U\/ml (on ß-glucan at 40oC) and pectinase \u0026lt; 0.07 U\/ml (on pectin at 40oC). \u003cbr\u003e\r\n\u003cbr\u003e\r\nNote: This product has been purified to remove a-galactosidase, ß-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).\u003cbr\u003e\r\n\u003cbr\u003e\r\nFructanase and sucrase (E-SUCR) are used in are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54333591486755,"sku":"MEGAE-FRMXLQ","price":604.0,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-FRMXLQ.jpg?v=1785853848"},{"product_id":"p-xylnbe-10g-xylan-beechwood-10g","title":"P-XYLNBE-10G Xylan (Beechwood) 10g","description":"\u003cp\u003eHighly purified xylan from beechwood for use in research, biochemical enzyme assays and analytical testing applications.\u003cbr\u003e\r\n\u003cbr\u003e\r\nSuitable as a replacement for birchwood xylan as a substrate for ß-xylanase in DNSA reducing sugar assay.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54748652142883,"sku":"MEGAP-XYLNBE-10G","price":405.4,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/P-XYLNBE-10G.jpg?v=1790298923"},{"product_id":"e-isamy-isoamylase-glycogen-6-glucanohydrolase","title":"E-ISAMY Isoamylase (Glycogen 6-glucanohydrolase)","description":"\u003cp\u003ePure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and analytical testing applications. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.\u003cbr\u003e\r\n\u003cbr\u003e\r\nPlease refer to E-ISAMYHP for high purity enzyme suitable for use in starch structural research.\u003c\/p\u003e","brand":"Megazyme","offers":[{"title":"Default Title","offer_id":54777168658723,"sku":"MEGAE-ISAMY","price":642.31,"currency_code":"NZD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/files\/E-ISAMY.jpg?v=1790298925"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0597\/5896\/9012\/collections\/ONELAB-FINAL-LOGO-B_9e023b0c-20e7-469a-944a-8b6adc9d9a96.jpg?v=1756937301","url":"https:\/\/onelab.co.nz\/collections\/enzymes.oembed","provider":"Onelab","version":"1.0","type":"link"}